Lightfoot E N, Moscariello J S
Department of Chemical and Biological Engineering, University of Wisconsin, Madison, USA.
Biotechnol Bioeng. 2004 Aug 5;87(3):259-73. doi: 10.1002/bit.20111.
Here we review key applications of separation technology in applied biology. We first sketch out the field as a whole, but then narrow our scope to the processing of fermentation products, particularly to high-value biologicals such as proteins and nucleotides. We go on to provide a qualitative overview describing the importance and general nature of this large field, major trends, and the strategies that have proven most fruitful in evolving effective separation and purification processes. We then give a detailed description of individual separations equipment and the principles governing their operation. We concentrate throughout on making the available literature accessible to the reader; we provide what is hoped to be a representative set of basic references. However, these references, in turn, include some that suggest promising new developments as well as a number of more specialized reviews. We hope that our overall result provides the reader with access to the most relevant literature.
在此，我们回顾了分离技术在应用生物学中的关键应用。我们首先勾勒出整个领域的概况，但随后将范围缩小到发酵产物的加工，特别是高价值生物制品，如蛋白质和核苷酸。接着，我们进行定性概述，描述这个广阔领域的重要性、一般性质、主要趋势以及在发展有效的分离和纯化过程中已被证明最有成效的策略。然后，我们详细描述了各个分离设备及其操作原理。我们始终专注于让读者能够获取现有文献；我们提供了一组希望具有代表性的基础参考文献。然而，这些参考文献反过来又包括一些暗示有前景的新发展的文献以及一些更专业的综述。我们希望我们的总体成果能让读者获取到最相关的文献。
