Shang Ya-Zhen, Qin Bo-Wen, Cheng Jian-Jun, Miao Hong
Institute of Chinese Traditional Medicine, Chengde Medical College, Chengde 067000, China.
Phytother Res. 2006 Jan;20(1):53-7. doi: 10.1002/ptr.1802.
Reactive oxygen species (ROS) are important mediators in a number of neurodegenerative diseases, such as Alzheimer's disease (AD) and Parkinson's disease (PD). The neuroprotective effects of flavonoids from the stems and leaves of Scutellaria baicalensis Georgi (SSF) against hydrogen peroxide (H2O2)-induced rat pheochromocytoma line PC12 injury were evaluated by cell lesion, free radicals and ATPase disorders. Following a 30 min exposure of the cells to H2O2 (100 microm), a marked decrease in cell survival and activity of superoxide dismutase (SOD) and Na+-K+-ATPase as well as an increase of malondialdehyde (MDA) production and lactate dehydrogenase (LDH) release were observed. Pretreatment of the cells with SSF (18-76 microg/mL) prior to H2O2 exposure notably elevated the cell survival and activity of SOD and Na+-K+-ATPase, and lowered the MDA level and LDH release. Neuroprotection by SSF was also observed in animal models. The present results indicated that SSF exerts neuroprotective effects against H2O2 toxicity, which might be of importance and might contribute to its clinical efficacy for the treatment of neurodegenerative disease.
活性氧（ROS）是许多神经退行性疾病（如阿尔茨海默病（AD）和帕金森病（PD））中的重要介质。通过细胞损伤、自由基和ATP酶紊乱评估了黄芩茎叶黄酮（SSF）对过氧化氢（H2O2）诱导的大鼠嗜铬细胞瘤细胞系PC12损伤的神经保护作用。在细胞暴露于H2O2（100微摩尔）30分钟后，观察到细胞存活率、超氧化物歧化酶（SOD）和钠钾ATP酶活性显著降低，丙二醛（MDA）生成量和乳酸脱氢酶（LDH）释放量增加。在H2O2暴露前用SSF（18 - 76微克/毫升）预处理细胞，显著提高了细胞存活率、SOD和钠钾ATP酶活性，并降低了MDA水平和LDH释放量。在动物模型中也观察到了SSF的神经保护作用。目前的结果表明，SSF对H2O2毒性具有神经保护作用，这可能具有重要意义，并可能有助于其治疗神经退行性疾病的临床疗效。
