利用磁性载体检测和操控生物分子。

Detection and manipulation of biomolecules by magnetic carriers.

作者信息

Brzeska M, Panhorst M, Kamp P B, Schotter J, Reiss G, Pühler A, Becker A, Brückl H

机构信息

Department of Physics, University of Bielefeld, Universitätsstrasse 25, 33615 Bielefeld, Germany.

出版信息

J Biotechnol. 2004 Aug 26;112(1-2):25-33. doi: 10.1016/j.jbiotec.2004.04.018.

DOI:10.1016/j.jbiotec.2004.04.018

PMID:15288938

Abstract

The detection and manipulation of single molecules on a common platform would be of great interest for basic research of biological or chemical systems. A promising approach is the application of magnetic carriers. The principles are demonstrated in this contribution. It is shown that paramagnetic beads can be detected by highly sensitive magnetoresistive sensors yielding a purely electronic signal. Different configurations are discussed. The capability of the sensors to detect even single markers is demonstrated by a model experiment. In addition, the paramagnetic beads can be used as carriers for biomolecules. They can be manipulated on-chip via currents running through specially designed line patterns. Thus, magnetic markers in combination with magnetoresistive sensors are a promising choice for future integrated lab-on-a-chip systems.

摘要

在一个通用平台上对单分子进行检测和操控，对于生物或化学系统的基础研究将具有极大的吸引力。一种很有前景的方法是应用磁性载体。本文阐述了相关原理。结果表明，顺磁珠可被高灵敏度磁阻传感器检测到，并产生纯电子信号。文中讨论了不同的配置。通过一个模型实验展示了传感器检测单个标记物的能力。此外，顺磁珠可用作生物分子的载体。它们可通过流经特殊设计线路图案的电流在芯片上进行操控。因此，磁性标记物与磁阻传感器相结合，对于未来的集成芯片实验室系统来说是一个很有前景的选择。