Safarik Ivo, Safarikova Mirka
Laboratory of Biochemistry and Microbiology, Institute of Landscape Ecology, Academy of Sciences, Na Sadkach 7, 370 05 Ceske Budejovice, Czech Republic.
Biomagn Res Technol. 2004 Nov 26;2(1):7. doi: 10.1186/1477-044X-2-7.
Isolation and separation of specific molecules is used in almost all areas of biosciences and biotechnology. Diverse procedures can be used to achieve this goal. Recently, increased attention has been paid to the development and application of magnetic separation techniques, which employ small magnetic particles. The purpose of this review paper is to summarize various methodologies, strategies and materials which can be used for the isolation and purification of target proteins and peptides with the help of magnetic field. An extensive list of realised purification procedures documents the efficiency of magnetic separation techniques.
特定分子的分离和提纯几乎应用于生物科学和生物技术的所有领域。可以采用多种方法来实现这一目标。近年来，人们越来越关注利用小磁珠的磁分离技术的开发和应用。这篇综述文章的目的是总结各种方法、策略和材料，借助磁场用于分离和提纯目标蛋白质和肽。大量已实现的提纯程序证明了磁分离技术的有效性。
