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鲁氏毛孢子菌对缩水甘油基芳基醚的生物催化拆分：细胞/底物比例影响(R)-环氧化物的光学纯度。

Biocatalytic resolution of glycidyl aryl ethers by Trichosporon loubierii : cell/substrate ratio influences the optical purity of (R) - epoxides.

作者信息

Xu Yi, Xu Jian-He, Pan Jiang, Tang Yan-Fa

机构信息

Laboratory of Biocatalysis and Bioprocessing, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, P.R. China.

出版信息

Biotechnol Lett. 2004 Aug;26(15):1217-21. doi: 10.1023/B:BILE.0000036598.35494.de.

DOI:10.1023/B:BILE.0000036598.35494.de
PMID:15289677
Abstract

Glycidyl aryl ethers were resolved by using lyophilized cells of Trichosporon loubierii ECU1040 having epoxide hydrolase activity. The activity and enantioselectivity depended on the structure of the aryl group. Different cell/substrate ratios also influenced the optical purity of remaining substrate. An additional stability test of the whole-cell enzyme suggests that rapid deactivation of the epoxide hydrolase was the potential reason. (R)-Epoxides were prepared in gram amounts with optical purity of 87% - 99% ee.

摘要

利用具有环氧水解酶活性的娄伯毕赤酵母ECU1040冻干细胞拆分缩水甘油芳基醚。活性和对映体选择性取决于芳基的结构。不同的细胞/底物比例也会影响剩余底物的光学纯度。全细胞酶的额外稳定性测试表明，环氧水解酶的快速失活是潜在原因。以克量制备了光学纯度为87%-99%ee的(R)-环氧化物。

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