利用假单胞菌属细菌的全细胞通过环氧水解酶催化拆分3-苯基缩水甘油酸乙酯
Epoxide hydrolase-catalyzed resolution of ethyl 3-phenylglycidate using whole cells of Pseudomonas sp.
作者信息
Li Congfa, Liu Qiao, Song Xin, Di Ding, Ji Aiguo, Qu Yinbo
机构信息
State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, PR. China.
出版信息
Biotechnol Lett. 2003 Dec;25(24):2113-6. doi: 10.1023/b:bile.0000007078.83362.a0.
Abstract
A Pseudomonas sp. was isolated with enantioselective epoxide hydrolase activity to ethyl 3-phenylglycidate. Cells grown on sucrose and suspended in 10% (v/v) dimethyl formamide as co-solvent produced (2R,3S) ethyl 3-phenylglycidate with 95% ee and 26% yield in 12 h from 0.2% (w/v) of the racemate.
摘要
分离出一株对3-苯基缩水甘油酸乙酯具有对映选择性环氧水解酶活性的假单胞菌属细菌。在蔗糖上生长并悬浮于10%（v/v）二甲基甲酰胺作为助溶剂的细胞，在12小时内从0.2%（w/v）的外消旋体中产生了ee值为95%、产率为26%的（2R,3S）-3-苯基缩水甘油酸乙酯。
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