Helliwell J R, Ealick S, Doing P, Irving T, Szebenyi M
Department of Chemistry, University of Manchester, England.
Acta Crystallogr D Biol Crystallogr. 1993 Jan 1;49(Pt 1):120-8. doi: 10.1107/S0907444992006747.
Synchrotron radiation has been used extensively to overcome a variety of technical challenges involved in data collection from macromolecular crystals. The next generation of such sources offer a higher brilliance at much shorter wavelengths than hitherto available. Hence, the quality of X-ray diffraction data from crystals of biological macromolecules will be further improved in terms of reduced systematic and random errors, in conjunction with a very high degree of completeness of, and multiple measurements within, the data set. Real data sets should be able to approach closely the quality of ideal data sets. Tests at CHESS are described of the feasibility of recording protein crystal diffraction patterns at ultra-short wavelengths (lambda = 0.3 A) and very-short wavelengths (lambda = 0.5 A), in monochromatic rotating crystal geometry.
同步辐射已被广泛用于克服从大分子晶体收集数据时所涉及的各种技术挑战。下一代此类光源在比以往更短的波长下具有更高的亮度。因此，来自生物大分子晶体的X射线衍射数据的质量将在减少系统误差和随机误差方面得到进一步提高，同时数据集具有非常高的完整性并能进行多次测量。实际数据集应能够非常接近理想数据集的质量。本文描述了在CHESS上进行的测试，即在单色旋转晶体几何结构中，在超短波长（λ = 0.3 Å）和极短波长（λ = 0.5 Å）下记录蛋白质晶体衍射图案的可行性。
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