Gall Yvonne, Woitag Tanja, Bauer Burkhard, Sidibe Issa, McDermott John, Mehlitz Dieter, Clausen Peter-Henning
Institute for Parasitology and International Animal Health, Freie Universität Berlin, Koenigsweg 67, D-14163 Berlin, Germany.
Acta Trop. 2004 Sep;92(1):7-16. doi: 10.1016/j.actatropica.2004.04.003.
The aim of this study was to assess, whether polymerase chain reaction (PCR) allows sensitive screening of treatment failure suspicions in areas, where drug resistance against African animal trypanosomosis (AAT) appears to be a problem. PCR was used to detect trypanosome infections prior to, 14 and 28 days after controlled treatment of 738 cattle from 10 villages in Kénédougou, Burkina Faso with isometamidium chloride and diminazene aceturate. Using three sets of primers, PCR was three-four times more sensitive and better at species identification, than standard microscopic examination. The better sensitivity and species specificity of PCR have important advantages for drug resistance studies in the field.
本研究的目的是评估在对非洲动物锥虫病（AAT）出现耐药性问题的地区，聚合酶链反应（PCR）是否能对治疗失败的怀疑进行灵敏筛查。对来自布基纳法索凯内杜古10个村庄的738头牛用氯咪啶和乙酰氨基阿维菌素进行对照治疗，在治疗前、治疗后14天和28天，用PCR检测锥虫感染情况。与标准显微镜检查相比，使用三组引物的PCR在物种鉴定方面灵敏度高三到四倍，效果更好。PCR更高的灵敏度和物种特异性在该领域的耐药性研究中具有重要优势。
