Pipino Luca, Braglia Luca, Giovannini Annalisa, Fascella Giancarlo, Mercuri Antonio
CRA-FSO Research Unit for Floriculture and Ornamental Species, Sanremo, Imperia, Italy.
Methods Mol Biol. 2010;589:153-62. doi: 10.1007/978-1-60327-114-1_15.
With more than 450 species, Passiflora is the most important genus of the family Passifloraceae. It comprises many species grown for their edible fruits, for their high ornamental value, and further for the therapeutic properties. With their striking exotic flowers, they are of particular interest for the floriculture market. With the aim of evaluating the in vitro propagation of an Italian ornamental hybrid, axillary tendrils of Passiflora "Guglielmo Betto" M. Vecchia (P. incarnata L. x P. tucumanensis L.) were sterilized and placed in vitro. Direct shoot regeneration was achieved from young tendrils cultivated on MS medium containing, either 4.43 microM 6-benzylaminopurine (BAP) and 11.41 microM indoleacetic acid (IAA), or 49.20 microM 6-gamma-gamma-dimethylallylaminopurine (2iP) and 2.68 microM alpha-naphthalene acetic acid (NAA), respectively. In vitro shoot multiplication, rooting, and regenerated plant acclimatization protocols were established.
西番莲属有450多个物种，是西番莲科最重要的属。它包含许多因其可食用果实、高观赏价值以及治疗特性而种植的物种。凭借其引人注目的异域花朵，它们在花卉市场中格外受关注。为了评估一种意大利观赏杂交种的离体繁殖情况，对西番莲“古列尔莫·贝托”M. 韦基亚（西番莲 incarnata L. × 西番莲 tucumanensis L.）的腋生卷须进行消毒并置于离体环境中。在含有4.43微摩尔6 - 苄基腺嘌呤（BAP）和11.41微摩尔吲哚乙酸（IAA），或分别含有49.20微摩尔6 - γ - γ - 二甲基烯丙基氨基嘌呤（2iP）和2.68微摩尔α - 萘乙酸（NAA）的MS培养基上培养幼嫩卷须，实现了直接芽再生。建立了离体芽增殖、生根和再生植株驯化方案。
