Sokova O I, Kirichenko O P, Mukeria A F, Demidov L V, Chebotarev A N, Kopnin B P
Laboratory of Cytogenetics, All-Union Cancer Research Center AMS of the USSR, Moscow.
Cancer Genet Cytogenet. 1992 Jan;58(1):24-8. doi: 10.1016/0165-4608(92)90128-u.
Frequency and distribution of 5-fluorodeoxyuridine (5-FdU) plus caffeine-induced fragile sites on chromosomes of peripheral blood lymphocytes (PBL) from 10 patients with cutaneous melanoma were studied in comparison with 10 PBL samples from normal donors of corresponding sex and age. The total number of breaks showed a significant difference among individuals in both groups, however, the average frequencies of 5-FdU plus caffeine-induced, as well as spontaneous damages in PBL from melanoma patients, were higher than those from healthy volunteers. The analysis of the breakpoint distribution showed a statistically significant increase in the expression of several fragile sites. The highest enhancement was observed at 1p32 and 1p22 sites (p less than 0.001). Earlier, the increase in the expression of 1p32 fragile sites was reported for neuroblastoma patients. We believe that enhanced expression of fragile sites in 1p may play a yet-unknown pathogenetic role in the development of some neuroectodermal tumors.
研究了10例皮肤黑色素瘤患者外周血淋巴细胞（PBL）染色体上5-氟脱氧尿苷（5-FdU）加咖啡因诱导的脆性位点的频率和分布，并与10例相应性别和年龄的正常供体的PBL样本进行了比较。两组个体间的断裂总数存在显著差异，然而，黑色素瘤患者PBL中5-FdU加咖啡因诱导的以及自发损伤的平均频率高于健康志愿者。断裂点分布分析显示，几个脆性位点的表达有统计学意义的增加。在1p32和1p22位点观察到最高的增强（p小于0.001）。此前，有报道称神经母细胞瘤患者1p32脆性位点的表达增加。我们认为，1p中脆性位点的增强表达可能在某些神经外胚层肿瘤的发生中起尚未明确的致病作用。
