Carlring Jennifer, Barr Tom A, McCormick Adele L, Heath Andrew W
Infection and Immunity: Division of Genomic Medicine, F Floor, University of Sheffield Medical School, Beech Hill Rd, Sheffield S10 2RX, UK.
Vaccine. 2004 Sep 3;22(25-26):3323-8. doi: 10.1016/j.vaccine.2004.02.043.
Monoclonal antibodies against CD40, conjugated to antigen, act as potent immunological adjuvants for primary antibody responses. We show here that CD40mAbs can also act as strong adjuvants for memory antibody responses, and for T cell responses as measured by ex vivo T cell proliferation to antigen, and delayed type hypersensitivity. Interferon gamma secretion in response to antigen is also enhanced. Finally, the adjuvant effect of CD40mAbs for secondary antibody responses is transferred with T cells rather than B cells. CD40mAb apparently have potent adjuvant effects on both Th1-like cells, and on T cells able to promote B cell antibody production. It is possible that the adjuvant effects of CD40 are mediated at least in part, indirectly, through enhanced antigen presentation by specific B cells, to T cells.
与抗原偶联的抗CD40单克隆抗体可作为初次抗体应答的强效免疫佐剂。我们在此表明，CD40单克隆抗体也可作为记忆抗体应答以及T细胞应答的强效佐剂，T细胞应答通过体外T细胞对抗原的增殖以及迟发型超敏反应来衡量。对抗原的γ干扰素分泌也会增强。最后，CD40单克隆抗体对二次抗体应答的佐剂作用是由T细胞而非B细胞传递的。CD40单克隆抗体显然对Th1样细胞以及能够促进B细胞抗体产生的T细胞均具有强效佐剂作用。CD40的佐剂作用有可能至少部分是通过特定B细胞向T细胞增强抗原呈递而间接介导的。
