Kawai Kenji, Liu Ying, Ohnishi Kouhei, Oshima Syun-Ichirou
Department of Aquaculture, Fish Disease Laboratory, Faculty of Agriculture, Research Institute of Molecular Genetics, Kochi University, Nankoku, Kochi 783-8502, Japan.
Vaccine. 2004 Sep 3;22(25-26):3411-8. doi: 10.1016/j.vaccine.2004.02.026.
An effective vaccine against Edwardsiella tarda has not been reported, one of main reasons is the variation in its serotypes. This study aimed to develop an effective vaccine against different serotypes of E. tarda. A conserved 37 kDa outer membrane protein (OMP) of E. tarda was obtained by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE). It showed comprising several proteins by two-dimensional (2D) PAGE analysis and showed a high similarity to glyceraldehyde-3-phosphate dehydrogenase by N-terminal amino acid sequence analysis. Japanese flounder (Paralichthys olivaceus) vaccinated with the 37 kDa OMP was significantly protected against the infections by different serotypes of E. tarda. A specific antibody was also detected by using ELISA. This study suggests that the 37 kDa OMP is an effective potent vaccine candidate against different serotypes of E. tarda.
尚未有针对迟缓爱德华氏菌的有效疫苗报道，主要原因之一是其血清型存在变异。本研究旨在开发一种针对不同血清型迟缓爱德华氏菌的有效疫苗。通过十二烷基硫酸钠（SDS）-聚丙烯酰胺凝胶电泳（PAGE）获得了迟缓爱德华氏菌一种保守的37 kDa外膜蛋白（OMP）。二维（2D）PAGE分析显示它由几种蛋白质组成，N端氨基酸序列分析表明其与甘油醛-3-磷酸脱氢酶具有高度相似性。用37 kDa OMP免疫的牙鲆（Paralichthys olivaceus）对不同血清型迟缓爱德华氏菌的感染具有显著的抵抗力。通过酶联免疫吸附测定（ELISA）也检测到了特异性抗体。本研究表明，37 kDa OMP是针对不同血清型迟缓爱德华氏菌的一种有效的潜在疫苗候选物。
