Mamede Fabrício V, Laus José L, Cabral Vânia P, Vicenti Felipe A M, Barbieri-Neto José
College of Agricultural and Veterinarian Sciences, São Paulo State University, UNESP - Jaboticabal, São Paulo, Brazil.
Vet Ophthalmol. 2004 Sep-Oct;7(5):335-42. doi: 10.1111/j.1463-5224.2004.04026.x.
To possibly reduce postoperative adhesions that occur after ocular myoplasties, we investigated the topical effects of 0.04% mitomycin C on the repaired areas of the medial rectus muscle using an equine renal capsule preserved in 98% glycerin for reinforcement of the sutures. Twenty-four rabbits, divided into two groups of 12 animals each [untreated (control) and treated group (MMC)], were submitted to surgical rupture of the medial rectus muscle of one eye and repair of the defect 24 h later with sutures and an equine renal capsule. Post-operative prophylactic treatment of the two groups consisted of the administration of eye drops containing neomycin, polymyxin B and dexamethasone at regular 6-h intervals for eight consecutive days and daily rinsing with physiological saline. MMC animals received additional treatment with topical 0.04% mitomycin C every 6 h for 14 consecutive days. Slit lamp biomicroscopy showed greater irritation of the ocular surface in MMC animals during the first days post operatively. Adhesions were observed at 15 and 30 days of assessment in the two groups, but were more extensive in control animals at 60 days. Histopathology revealed inflammatory exudation in both groups, which was greater in MMC animals. Mitomycin C (0.04%) instilled at 6-h intervals for 14 consecutive days reduced the occurrence of fibrosis in the myoplastic areas. However, the equine renal capsule was found to be of little benefit for the reinforcement of myoplasties.
为了尽可能减少眼肌成形术后发生的术后粘连，我们使用保存在98%甘油中的马肾包膜来加强缝合，研究了0.04%丝裂霉素C对内侧直肌修复区域的局部作用。将24只兔子分为两组，每组12只动物[未治疗（对照组）和治疗组（MMC）]，对一只眼睛的内侧直肌进行手术切断，并在24小时后用缝线和马肾包膜修复缺损。两组术后的预防性治疗包括连续八天每隔6小时滴注含有新霉素、多粘菌素B和地塞米松的眼药水，并每天用生理盐水冲洗。MMC组的动物连续14天每隔6小时额外接受一次局部0.04%丝裂霉素C治疗。裂隙灯生物显微镜检查显示，MMC组动物在术后头几天眼表的刺激更明显。在评估的第15天和第30天，两组均观察到粘连，但在60天时对照组的粘连更广泛。组织病理学显示两组均有炎性渗出，MMC组动物的炎性渗出更严重。连续14天每隔6小时滴注0.04%丝裂霉素C可减少肌成形术区域纤维化的发生。然而，发现马肾包膜对加强肌成形术几乎没有益处。
