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Interaction of Wnt-1 proteins with the binding protein BiP.

作者信息

Kitajewski J, Mason J O, Varmus H E

机构信息

Department of Microbiology, University of California, San Francisco 94143-0502.

出版信息

Mol Cell Biol. 1992 Feb;12(2):784-90. doi: 10.1128/mcb.12.2.784-790.1992.

DOI:10.1128/mcb.12.2.784-790.1992
PMID:1531088
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC364299/
Abstract

The mouse Wnt-1 gene, a target for insertional activation in mouse mammary tumor virus-induced mammary tumors, encodes poorly secreted, cysteine-rich glycoproteins required for proper central nervous system development. We have been analyzing the biosynthesis of Wnt-1 proteins in several cell lines that express Wnt-1 cDNA from heterologous promoters. A protein of 78 kDa was found to be associated with the intracellular forms of Wnt-1 proteins in mammalian and avian cells by using multiple antisera against Wnt-1 proteins. We have identified p78 as the binding protein BiP with anti-BiP antisera and by its release from Wnt-1 immunoprecipitates upon incubation with MgCl2 and ATP. Experiments with a Wnt-1 mutant that lacks the sequence encoding the signal peptide indicates that Wnt-1 proteins must enter the secretory pathway in order to interact with BiP. We demonstrate that Wnt-1 proteins are associated with BiP in cells in which active Wnt-1 proteins are produced, such as a cultured mammary epithelial cell line and Wnt-1 transgenic mouse mammary tumor cells. The association of Wnt-1 proteins with BiP may be a factor in determining the efficiency of secretion of Wnt-1 gene products.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1402/364299/0459f3012912/molcellb00026-0366-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1402/364299/22d27d745ff8/molcellb00026-0364-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1402/364299/2566e700c888/molcellb00026-0365-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1402/364299/6bae188f2864/molcellb00026-0365-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1402/364299/c87a6246dd72/molcellb00026-0366-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1402/364299/0459f3012912/molcellb00026-0366-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1402/364299/22d27d745ff8/molcellb00026-0364-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1402/364299/2566e700c888/molcellb00026-0365-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1402/364299/6bae188f2864/molcellb00026-0365-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1402/364299/c87a6246dd72/molcellb00026-0366-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1402/364299/0459f3012912/molcellb00026-0366-b.jpg

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The int-1 proto-oncogene products are glycoproteins that appear to enter the secretory pathway.原癌基因int-1的产物是糖蛋白，似乎进入分泌途径。
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