Eggert Lori S, Maldonado Jesús E, Fleischer Robert C
Department of Systematic Biology, National Museum of Natural History, Smithsonian Institution, Washington, DC 20008-0551, USA.
Methods Enzymol. 2005;395:73-87. doi: 10.1016/S0076-6879(05)95006-4.
Noninvasive sampling is very attractive to field biologists and has tremendous potential for studying secretive species and being a cost-effective method of increasing sample sizes in studies of large, dangerous animals. Extracting DNA from noninvasively collected samples can be challenging, and the methods have been developed mainly through modification of previously developed protocols for other sample types. We present the most commonly used methods along with modifications used by some researchers to deal with the problem of coextraction of polymerase chain reaction (PCR) inhibitors. Although it is difficult to generalize about which methods should be used on particular sample types, we discuss the success of the methods in studies to date. We close with general suggestions for dealing with potential problems associated with the analysis of DNA obtained from noninvasively collected samples.
非侵入性采样对野外生物学家极具吸引力，在研究隐秘物种以及作为一种增加大型危险动物研究样本量的经济有效方法方面具有巨大潜力。从非侵入性采集的样本中提取DNA可能具有挑战性，相关方法主要是通过对先前针对其他样本类型开发的方案进行修改而得以发展。我们介绍了最常用的方法以及一些研究人员为应对聚合酶链反应（PCR）抑制剂共提取问题所采用的修改方法。尽管很难一概而论地说明针对特定样本类型应使用哪种方法，但我们讨论了这些方法在迄今为止的研究中的成功情况。最后，我们针对处理与分析从非侵入性采集样本中获得的DNA相关的潜在问题给出了一般性建议。
