[藏族人群7个Y染色体短串联重复序列基因座的遗传多态性及单倍型]

[The genetic polymorphism of 7 Y-STR loci and haplotype in Tibetan population].

作者信息

Zhao Dong, Wang Bao-jie, Ding Mei

机构信息

Department of forensic medicine, China Medical University, Shenyang 110001, China.

出版信息

Fa Yi Xue Za Zhi. 2004;20(2):88-91.

PMID:15311522

Abstract

OBJECTIVE

To investigate the genetic polymorphism of 7 Y-STR loci and haplotypes in Tibetan population.

METHODS

The DNA samples from 67 Tibetan men were analyzed by PCR and the denatured PAGE followed by silver staining.

RESULTS

25 alleles were identified for 6 loci including DYS393, DYS389 I, DYS19, DYS390, DYS389 II and DYS392, 22 alleles were identified for DYS385, the frequency distributions were between 0.0149-0.5075, the DP value distributions were between 0.5075-0.8991, the DP value of DYS 385 was the highest. 51 haplotypes were identified for the YH6 system which consisted of the 7 loci, the DP value of this system was 0.9887, the alleles for DYS393 and DYS389 II were few, it followed that perhaps Tibetan population had met with bottleneck event in their evolution process.

CONCLUSION

The 7 STR loci above belong to loci of high discriminating ability, the haplotypes are highly polymorphic.

摘要

目的

研究藏族人群7个Y-STR基因座及单倍型的遗传多态性。

方法

采用PCR扩增、变性聚丙烯酰胺凝胶电泳及银染技术，对67名藏族男性的DNA样本进行分析。

结果

在DYS393、DYS389 I、DYS19、DYS390、DYS389 II和DYS392这6个基因座共检出25个等位基因，DYS385检出22个等位基因，频率分布在0.0149 - 0.5075之间，DP值分布在0.5075 - 0.8991之间，其中DYS385的DP值最高。由这7个基因座组成的YH6系统共检出51种单倍型，该系统的DP值为0.9887，DYS393和DYS389 II的等位基因较少，提示藏族人群在进化过程中可能经历过瓶颈事件。