Zhao Dong, Wang Bao-jie, Ding Mei
Department of forensic medicine, China Medical University, Shenyang 110001, China.
Fa Yi Xue Za Zhi. 2004;20(2):88-91.
To investigate the genetic polymorphism of 7 Y-STR loci and haplotypes in Tibetan population.
The DNA samples from 67 Tibetan men were analyzed by PCR and the denatured PAGE followed by silver staining.
25 alleles were identified for 6 loci including DYS393, DYS389 I, DYS19, DYS390, DYS389 II and DYS392, 22 alleles were identified for DYS385, the frequency distributions were between 0.0149-0.5075, the DP value distributions were between 0.5075-0.8991, the DP value of DYS 385 was the highest. 51 haplotypes were identified for the YH6 system which consisted of the 7 loci, the DP value of this system was 0.9887, the alleles for DYS393 and DYS389 II were few, it followed that perhaps Tibetan population had met with bottleneck event in their evolution process.
The 7 STR loci above belong to loci of high discriminating ability, the haplotypes are highly polymorphic.
研究藏族人群7个Y-STR基因座及单倍型的遗传多态性。
采用PCR扩增、变性聚丙烯酰胺凝胶电泳及银染技术，对67名藏族男性的DNA样本进行分析。
在DYS393、DYS389 I、DYS19、DYS390、DYS389 II和DYS392这6个基因座共检出25个等位基因，DYS385检出22个等位基因，频率分布在0.0149 - 0.5075之间，DP值分布在0.5075 - 0.8991之间，其中DYS385的DP值最高。由这7个基因座组成的YH6系统共检出51种单倍型，该系统的DP值为0.9887，DYS393和DYS389 II的等位基因较少，提示藏族人群在进化过程中可能经历过瓶颈事件。
上述7个STR基因座属于高识别能力基因座，单倍型具有高度多态性。
