[中国汉族和日本人群7个Y染色体短串联重复序列基因座及单倍型的遗传多态性与群体差异]

[Genetic polymorphism and the population difference of 7 Y-STR loci and haplotypes in Chinese Han and Japanese populations].

作者信息

Zhao Dong, Wang Bao-jie, Ding Mei

机构信息

Department of Forensic Medicine, China Medical University, Shenyang 110001, China.

出版信息

Fa Yi Xue Za Zhi. 2003;19(3):143-8.

PMID:13677315

Abstract

OBJECTIVE

To investigate the genetic polymorphism of 7 Y-STR loci and haplotypes and analyze their population difference.

METHODS

The DNA samples from 45 Chinese Han and 59 Japanese men were analyzed by PCR and the denatured PAGE followed by silver staining.

RESULTS

33 alleles were identified for 6 loci including DYS393, DYS389 I, DYS19, DYS390, DYS389 II and DYS392, 39 alleles were identified for DYS 385, the frequency distributions were between 0.0169-0.6444, the DP value distributions of them were between 0.5406-0.9579, the DP value of DYS 385 was the highest. With the comparison of data of the 7 loci, significant difference in genetics was observed between the two populations(P < 0.05). There were 95 haplotypes that consisted of the 7 loci, Chinese Han had 41 haplotypes, DP value 0.9960, Japanese population had 54 haplotypes, DP value 0.9965, no common haplotypes were found between them.

CONCLUSION

The 7 STR loci above belong to loci of high discriminating ability, the haplotypes are highly polymorphic and their ethical character is apparent.

摘要

目的

研究7个Y染色体短串联重复序列（Y-STR）基因座的遗传多态性及单倍型，并分析其群体差异。

方法

采用聚合酶链反应（PCR）、变性聚丙烯酰胺凝胶电泳（PAGE）及银染技术，对45名中国汉族男性和59名日本男性的DNA样本进行分析。

结果

在DYS393、DYS389 I、DYS19、DYS390、DYS389 II和DYS392这6个基因座共检出33个等位基因，DYS385基因座检出39个等位基因，其频率分布在0.0169 - 0.6444之间，个体识别力（DP）值分布在0.5406 - 0.9579之间，其中DYS385基因座的DP值最高。对7个基因座的数据进行比较，两个群体间存在显著的遗传学差异（P < 0.05）。7个基因座组成95种单倍型，中国汉族有41种单倍型，DP值为0.9960，日本群体有54种单倍型，DP值为0.9965，两者之间未发现共同单倍型。