Báo S N, Gonçalves Simões D, Lino-Neto J
Department of Cellular Biology, Institute of Biological Sciences, University of Brasília, DF, Brazil.
J Submicrosc Cytol Pathol. 2004 Jan;36(1):23-8.
The spermatozoa of Exomalopsis auropilosa and Paratetrapedia (Lophopedia) sp. are long and slender, measuring about 374 microm and 370 microm in length of which the head region measures approximately 25.8 and 28.3 microm, respectively. The head consists of an acrosome formed by an acrosomal vesicle covering a perforatorium, which presents a paracrystalline organization in Paratetrapedia (Lophopedia) sp. and a nucleus. This latter measures about 24 microm in Exomalopsis auropilosa and 27 microm in Paratetrapedia (Lophopedia) sp., and has compact chromatin. The nucleus is attached to the flagellum by an electron dense material and centriolar adjunct is observed between it and smaller mitochondrial derivative. In this flagellar region only one accessory body is observed, which occurs between the larger mitochondrial derivative and the axoneme. The flagellum consists in a typical axoneme, 9+9+2 microtubules, two mitochondrial derivatives and two accessory bodies. The two mitochondrial derivatives are asymmetric in both length and diameter, and paracrystalline material appears only in the larger mitochondrial derivative. The structure and ultrastructure of the spermatozoa of the bee species here described are similar to the majority of sperm found in the other Hymenoptera and may be a contribution for future phylogenetic analysis of Apidae.
金毛外孔蜂（Exomalopsis auropilosa）和拟四节蜂属（Lophopedia）某种蜜蜂的精子细长，长度分别约为374微米和370微米，其中头部区域分别约为25.8微米和28.3微米。头部由顶体和细胞核组成，顶体由顶体囊形成，覆盖着穿孔器，在拟四节蜂属（Lophopedia）某种蜜蜂中呈现出准晶体结构。在金毛外孔蜂中，细胞核长约24微米，在拟四节蜂属（Lophopedia）某种蜜蜂中长约27微米，且染色质致密。细胞核通过电子致密物质与鞭毛相连，在细胞核与较小的线粒体衍生物之间观察到中心粒附属物。在这个鞭毛区域仅观察到一个辅助体，它位于较大的线粒体衍生物和轴丝之间。鞭毛由典型的轴丝（9+9+2微管）、两个线粒体衍生物和两个辅助体组成。这两个线粒体衍生物在长度和直径上均不对称，准晶体物质仅出现在较大的线粒体衍生物中。此处描述的蜜蜂物种精子的结构和超微结构与其他膜翅目昆虫中发现的大多数精子相似，可能有助于未来对蜜蜂科进行系统发育分析。
