Instituto de Ciências Biológicas e da Saúde, Universidade Federal de Viçosa/Campus Rio Paranaíba, Minas Gerais, Brazil.
Microsc Res Tech. 2012 Jan;75(1):74-80. doi: 10.1002/jemt.21027. Epub 2011 Oct 22.
Morphology of spermatozoa in bees has provided promising results for phylogenetic analyses. In this work, the structure and ultrastructure of spermatozoa from Thygater (Thygater) analis and Melitoma segmentaria were characterized and the synapomorphies shared in the family Apidae are discussed. In these species, spermatozoa bundles which are undone in the seminal vesicle possess, on average, 50 cells. Spermatozoa consist of a head and a flagellar region. The head includes an acrosome containing the perforatorium, covered by the acrosomal vesicle and a nucleus. The flagellum is formed by two mitochondrial derivatives, which are asymmetric in diameter and length, with one centriolar adjunct, one axoneme (9 + 9 + 2), and two accessory bodies. In cross section the centriolar adjunct is asymmetric and the accessory bodies are triangular in shape. In the distal region of the flagellum, the derivative terminates before the axoneme and the small derivative terminates first. The axoneme is gradually disorganized and the accessories microtubules are the last to terminate. In these two species, spermatozoa share diverse synapomorphies with those of other bee species previously described in the literature, which allows for the establishment of a morphological pattern for spermatozoa of the family Apidae.
蜜蜂精子的形态为系统发育分析提供了有希望的结果。在这项工作中，对 Thygater (Thygater) analis 和 Melitoma segmentaria 的精子结构和超微结构进行了描述，并讨论了在 Apidae 科中共享的同源特征。在这些物种中，在精囊内解开的精子束平均具有 50 个细胞。精子由头部和鞭毛区组成。头部包括一个含有穿孔器的顶体，由顶体囊和核覆盖。鞭毛由两个不对称直径和长度的线粒体衍生物组成，有一个中心粒附属物、一个轴丝（9+9+2）和两个附属体。在横截面上，中心粒附属物不对称，附属体呈三角形。在鞭毛的远端，衍生物在轴丝之前终止，小衍生物首先终止。轴丝逐渐解体，附属微管最后解体。在这两个物种中，精子与文献中先前描述的其他蜜蜂物种共享多种同源特征，这为 Apidae 科精子的形态模式的建立提供了依据。
