Zama U, Brito P, Lino-Neto J, Campos L A O, Doldero H, Báo S N
Department of Cellular Biology, Institute of Biological Sciences, University of Brasilia, DF, Brazil.
J Submicrosc Cytol Pathol. 2005 Nov;37(3-4):313-21.
The morphology of spermatozoon of Sceliphron fistularium is very similar to that described for bees. In particular, the response to E-PTA stains is similar to that observed in corbiculated Apidae, especially Meliponini bees. Spermatozoa measure 285 microm and are composed of 1) a bilayered acrosome (acrosomal vesicle and perforatorium); 2) a homogeneous and compact nucleus; 3) a 9+9+2 axoneme; 4) a rod-shaped centriolar adjunct; 5) two asymmetrical mitochondrial derivatives with paracrystalline material exclusively in the larger one, and 6) two accessory bodies. Only the accessory microtubules of axoneme and the paracrystalline material are E-PTA positive. Comparison of S. fistularium sperm to data on Hymenoptera corroborates their proximity with bees.
瘘管泥蜂精子的形态与蜜蜂的描述非常相似。特别是，对E-PTA染色的反应与在群居蜜蜂科，尤其是无刺蜂属蜜蜂中观察到的相似。精子长285微米，由以下部分组成：1）双层顶体（顶体囊泡和穿孔器）；2）均匀致密的细胞核；3）9+9+2轴丝；4）杆状中心粒附属物；5）两个不对称的线粒体衍生物，只有较大的一个含有平行排列的晶体状物质，以及6）两个辅助体。只有轴丝的辅助微管和平行排列的晶体状物质对E-PTA呈阳性反应。将瘘管泥蜂的精子与膜翅目数据进行比较，证实了它们与蜜蜂的亲缘关系。
