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构巢曲霉中的基因克隆：异柠檬酸裂解酶基因（acuD）的分离

Gene cloning in Aspergillus nidulans: isolation of the isocitrate lyase gene (acuD).

作者信息

Ballance D J, Turner G

出版信息

Mol Gen Genet. 1986 Feb;202(2):271-5. doi: 10.1007/BF00331649.

DOI:10.1007/BF00331649
PMID:3010050
Abstract

An Aspergillus nidulans gene library was constructed in a high-frequency transformation vector, pDJB3, based on the Neurospora crassa pyr4 gene. This gene library was used to isolate the structural gene for isocitrate lyase (acuD) by complementation of a deficiency mutation following transformation of A. nidulans. Plasmids rescued in Escherichia coli were able to transform five different A. nidulans acuD mutants. Transformation using plasmids containing the cloned fragment resulted in integration at the acuD locus in six of nine transformants.

摘要

基于粗糙脉孢菌pyr4基因，在高频转化载体pDJB3中构建了构巢曲霉基因文库。该基因文库用于通过转化构巢曲霉后对缺陷突变的互补作用来分离异柠檬酸裂解酶（acuD）的结构基因。在大肠杆菌中拯救的质粒能够转化五种不同的构巢曲霉acuD突变体。使用含有克隆片段的质粒进行转化，在九个转化体中有六个在acuD位点发生整合。

相似文献

1
Gene cloning in Aspergillus nidulans: isolation of the isocitrate lyase gene (acuD).构巢曲霉中的基因克隆：异柠檬酸裂解酶基因（acuD）的分离
Mol Gen Genet. 1986 Feb;202(2):271-5. doi: 10.1007/BF00331649.
2
Regulation of the expression of the isocitrate lyase gene (acuD) of Aspergillus nidulans.构巢曲霉异柠檬酸裂解酶基因（acuD）表达的调控
Mol Gen Genet. 1994 Feb;242(4):484-9. doi: 10.1007/BF00281801.
3
Characterization of the glyoxysomal isocitrate lyase genes of Aspergillus nidulans (acuD) and Neurospora crassa (acu-3).构巢曲霉（acuD）和粗糙脉孢菌（acu-3）乙醛酸循环体异柠檬酸裂解酶基因的特性分析
Curr Genet. 1992 Jan;21(1):43-7. doi: 10.1007/BF00318653.
4
Development of a high-frequency transforming vector for Aspergillus nidulans.构巢曲霉高频转化载体的构建
Gene. 1985;36(3):321-31. doi: 10.1016/0378-1119(85)90187-8.
5
Analysis of the regulation of the Aspergillus nidulans acuD gene, encoding isocitrate lyase, by construction of a hybrid promoter.通过构建杂合启动子分析构巢曲霉acuD基因（编码异柠檬酸裂合酶）的调控
Mol Gen Genet. 1994 Jun 15;243(6):654-9. doi: 10.1007/BF00279574.
6
Increased and decreased sensitivity to carbon catabolite repression of enzymes of acetate metabolism in mutants of Aspergillus nidulans.构巢曲霉突变体中乙酸代谢酶对碳分解代谢物阻遏的敏感性增加和降低
Mol Gen Genet. 1977 Nov 4;156(1):87-92. doi: 10.1007/BF00272256.
7
Cloning and characterization of the ornithine carbamoyltransferase gene from Aspergillus nidulans.构巢曲霉鸟氨酸氨甲酰基转移酶基因的克隆与特性分析
Gene. 1983 Nov;25(1):109-17. doi: 10.1016/0378-1119(83)90173-7.
8
Transformation of Aspergillus nidulans with a cloned, oligomycin-resistant ATP synthase subunit 9 gene.用克隆的抗寡霉素ATP合酶亚基9基因转化构巢曲霉。
Mol Gen Genet. 1986 Feb;202(2):265-70. doi: 10.1007/BF00331648.
9
Co-transformation with autonomously-replicating helper plasmids facilitates gene cloning from an Aspergillus nidulans gene library.与自主复制的辅助质粒共转化有助于从构巢曲霉基因文库中克隆基因。
Curr Genet. 1993 Dec;24(6):520-4. doi: 10.1007/BF00351716.
10
Cloning of Aspergillus nidulans DNA. I. Selection and analysis of recombinant plasmids capable of complementing pyrF, argIF and proAB mutations in Escherichia coli.构巢曲霉DNA的克隆。I. 能够互补大肠杆菌中pyrF、argIF和proAB突变的重组质粒的筛选与分析。
Acta Microbiol Pol. 1980;29(3):213-25.

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Eukaryot Cell. 2006 May;5(5):794-805. doi: 10.1128/EC.5.5.794-805.2006.
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Impact of a disruption of a pathway delivering copper to mitochondria on Podospora anserina metabolism and life span.将铜输送到线粒体的途径中断对嗜热栖热放线菌代谢和寿命的影响。
Eukaryot Cell. 2004 Feb;3(1):200-11. doi: 10.1128/EC.3.1.200-211.2004.
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Two classes of isocitrate lyase genes are expressed during late embryogeny and postgermination in Brassica napus L.

本文引用的文献

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A cosmid for selecting genes by complementation in Aspergillus nidulans: Selection of the developmentally regulated yA locus.通过互补作用在构巢曲霉中选择基因的 cosmid：发育调节的 yA 基因座的选择。
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Trans-complementable copy-number mutants of plasmid ColE1.质粒ColE1的反式可互补拷贝数突变体
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Regulation of the expression of the isocitrate lyase gene (acuD) of Aspergillus nidulans.构巢曲霉异柠檬酸裂解酶基因（acuD）表达的调控
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Isolation of the facA (acetyl-CoA synthetase) gene of Phycomyces blakesleeanus.布氏毛霉facA（乙酰辅酶A合成酶）基因的分离
Mol Gen Genet. 1994 Aug 2;244(3):278-86. doi: 10.1007/BF00285455.
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Isolation and characterisation of genes for sulphate activation and reduction in Aspergillus nidulans: implications for evolution of an allosteric control region by gene duplication.构巢曲霉中硫酸盐活化与还原相关基因的分离与鉴定：基因复制对变构控制区域进化的影响
Mol Gen Genet. 1995 May 20;247(4):423-9. doi: 10.1007/BF00293143.
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The Thom Award address. Industrial mycology and the new genetics.汤姆奖致辞。工业真菌学与新遗传学。
J Ind Microbiol. 1995 May;14(5):355-64. doi: 10.1007/BF01569951.
10
Genetic analysis of Aspergillus nidulans AmdS+ transformants.构巢曲霉AmdS+转化体的遗传分析。
Mol Gen Genet. 1986 Nov;205(2):312-7. doi: 10.1007/BF00430444.
Gene. 1983 Dec;26(2-3):205-21. doi: 10.1016/0378-1119(83)90191-9.
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Isolation of genomic clones containing the amdS gene of Aspergillus nidulans and their use in the analysis of structural and regulatory mutations.构巢曲霉amdS基因基因组克隆的分离及其在结构和调控突变分析中的应用。
Mol Cell Biol. 1983 Aug;3(8):1430-9. doi: 10.1128/mcb.3.8.1430-1439.1983.
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Studies on transformation of Escherichia coli with plasmids.大肠杆菌质粒转化的研究。
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Transformation of Aspergillus nidulans by using a trpC plasmid.利用trpC质粒对构巢曲霉进行转化。
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A rapid boiling method for the preparation of bacterial plasmids.一种制备细菌质粒的快速煮沸法。
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A reliable method for the recovery of DNA fragments from agarose and acrylamide gels.一种从琼脂糖凝胶和丙烯酰胺凝胶中回收DNA片段的可靠方法。
Anal Biochem. 1981 Apr;112(2):295-8. doi: 10.1016/0003-2697(81)90296-7.
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Transformation of Aspergillus nidulans by the orotidine-5'-phosphate decarboxylase gene of Neurospora crassa.用粗糙脉孢菌的乳清酸核苷-5'-磷酸脱羧酶基因转化构巢曲霉。
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The two-way selection of mutants and revertants in respect of acetate utilization and resistance to fluoro-acetate in Aspergillus nidulans.构巢曲霉在醋酸利用和对氟乙酸抗性方面突变体与回复突变体的双向选择。
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