Bowyer P, De Lucas J R, Turner G
Department of Molecular Biology and Biotechnology, University of Sheffield, UK.
Mol Gen Genet. 1994 Feb;242(4):484-9. doi: 10.1007/BF00281801.
Analysis of the promoter region of the acetate-induced isocitrate lyase gene (acuD) of Aspergillus nidulans is described. Transcription start sites were detected at positions -163, -170 and approximately -281 upstream of the ATG. Transcription analysis showed that the acuD gene is transcribed during growth on acetate but not on hexoses or glycerol. Expression of the acuD gene was studied under inducing and repressing conditions in cre+, creA, creB and creC mutant strains, showing that the creA(d)-1 mutation led to slight derepression of isocitrate lyase. Regulation of expression of the acuD gene was also studied using an in-frame fusion with the lacZ gene of Escherichia coli. Several deletions were made in order to identify the regions responsible for acetate induction and repression. A deletion of the -412 to -200 bp upstream region resulted in loss of all promoter activity and a smaller deletion within this region abolished most of the acetate inducibility.
本文描述了对构巢曲霉乙酸诱导型异柠檬酸裂解酶基因（acuD）启动子区域的分析。在起始密码子（ATG）上游-163、-170和大约-281位置检测到转录起始位点。转录分析表明，acuD基因在乙酸培养基上生长时转录，但在己糖或甘油培养基上不转录。在cre+、creA、creB和creC突变菌株的诱导和抑制条件下研究了acuD基因的表达，结果表明creA(d)-1突变导致异柠檬酸裂解酶略有去阻遏。还使用与大肠杆菌lacZ基因的读框融合研究了acuD基因的表达调控。进行了几次缺失操作以确定负责乙酸诱导和抑制的区域。上游-412至-200 bp区域的缺失导致所有启动子活性丧失，该区域内较小的缺失消除了大部分乙酸诱导性。
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