Lü Shu-qing, Xu Xiao-ping, Xia Fang, Wang Jian-min
Department of Hematology, Changhai Hospital of Second Military Medical University, Shanghai 200433, China.
Zhonghua Zhong Liu Za Zhi. 2004 Apr;26(4):196-200.
To study the molecular mechanism of tumorigenicity in nude mice of human leukemia cell lines.
K562-n, is a human leukemic cell line with much higher tumorigenecity in nude mice compared with the parental K562 cell line by repeated in vitro and in vivo passages. Genes differentially expressed between K562 and K562-n cells were analyzed by using DNA microarray technique.
Of 12 000 genes screened were differentially expressed significantly, among which 42 genes were up-regulated and 97 genes were down-regulated in K562-n cells. Eighty-five of the 139 genes have been registered in the GeneBank and 54 are unknown genes. The genes accessible from the GenBank include: 1. oncogenes and tumor-supressor genes, 2. genes related to transcription regulation, cell cycle and apoptosis, 3. genes related to cytoskeleton and cytokinetics, 4. genes related to metabolism and transport, 5. genes related to immune function. There were also some differentially expressed genes with mixed functions and some with unknown function differentially expressed.
There are many genes differentially expressed between K562-n and K562 cells. The high tumorigenicity in nude mice of human leukemia cell line K562-n might be related to its specific gene expression profile.
研究人白血病细胞系在裸鼠体内致瘤性的分子机制。
K562-n是一株人白血病细胞系，通过反复的体外和体内传代，其在裸鼠体内的致瘤性比亲代K562细胞系高得多。采用DNA微阵列技术分析K562和K562-n细胞之间差异表达的基因。
在筛选的12000个基因中，有139个基因差异表达显著，其中K562-n细胞中有42个基因上调，97个基因下调。这139个基因中有85个已在基因库中登记，54个为未知基因。从基因库中获得的基因包括：1. 癌基因和抑癌基因；2. 与转录调控、细胞周期和凋亡相关的基因；3. 与细胞骨架和细胞动力学相关的基因；4. 与代谢和转运相关的基因；5. 与免疫功能相关的基因。也有一些具有混合功能的差异表达基因以及一些功能未知的差异表达基因。
K562-n和K562细胞之间存在许多差异表达的基因。人白血病细胞系K562-n在裸鼠体内的高致瘤性可能与其特定的基因表达谱有关。
