Chen Li, Xu Xiao-ping, Wang Jian-min, Li Yao, Xu Hong, Lü Shu-qing, Fei Xin-hong, Huang Zheng-xia
Department of Hematology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China.
Zhonghua Zhong Liu Za Zhi. 2005 Apr;27(4):196-200.
To investigate the mechanism of multi-drug resistance of K562-n/VCR cell line with both bcr-abl and mdr-1 expressions by clustering analysis of differential gene expression profiles.
By DNA microarray technique, genes differentially expressed by K562-n/VCR and K562-n cell lines were identified and analyzed.
DNA microarray analysis of K562-n/VCR and K562-n cells was repeated three times and revealed 58 genes significantly differentially expressed among 12,800 genes arrayed. All but one was up-regulated in K562-n/VCR cells. The only gene down-regulated was a-myb. The up-regulated genes were MDR-associated genes, oncogenes, cytoskeleton, protein kinases and phosphatases, apoptotic and antiapoptotic factors, metabolism, transcriptional regulators associated with stress response, cell cycle checkpoint control, and genes for signal transduction proteins.
These results indicate that, besides MDR-associated genes, other known and unknown genes may also be involved in the mechanism of multi-drug resistance.
