Zhang Zhi, Liu Yan, Zhang Xiong, Xu Wei-Shi
Burn Institute of Shanghai, Rui Jin Hospital, Shanghai Second Medical University, Shanghai 200025, P. R. China.
Zhonghua Shao Shang Za Zhi. 2004 Apr;20(2):76-8.
To investigate the content of decorin and its mRNA expression in normal human skin and hyperplastic scars at different stages, so as to explore the relationship between the change of decorin and its synthesis.
Scar tissue samples from 22 patients undergoing scar excision and 10 specimens of normal skin or prepuce were obtained. The content and distribution of decorin in the tissue samples were determined with immunohistochemistry and Western blot, and the expression of decorin mRNA was detected by in situ hybridization.
The content of decorin was rich in the normal skin dermis with lower expression of the mRNA. In contrast, the decorin content was scarce in hyperplastic scars (HS) within 6 months, but increased gradually beginning from 7 to 12 months, and increased continuously for 13 to 36 months. There was no difference between the decorin content in normal skin and that in HS after 36 months (P > 0.05). Furthermore, the mRNA expression level in HS tissue was lower than that in normal skin within 6 months, but increased from 7 to 12 months. The mRNA expression continuously increased during 13 to 36 months and then returned to the level similar to that in normal skin thereafter.
The decrease of decorin in hyperplastic scar was resulted primarily from reduced synthesis. The increase in decorin level coincided with the time of scar tissue stabilization, which implied that the delayed appearance was correlated with the formation of HS.
研究核心蛋白聚糖的含量及其mRNA在正常人皮肤和不同阶段增生性瘢痕中的表达，以探讨核心蛋白聚糖变化与其合成之间的关系。
获取22例接受瘢痕切除患者的瘢痕组织样本以及10例正常皮肤或包皮样本。采用免疫组织化学和蛋白质印迹法测定组织样本中核心蛋白聚糖的含量和分布，通过原位杂交检测核心蛋白聚糖mRNA的表达。
核心蛋白聚糖在正常皮肤真皮中含量丰富，mRNA表达较低。相比之下，增生性瘢痕（HS）在6个月内核心蛋白聚糖含量稀少，但从7至12个月开始逐渐增加，并在13至36个月持续增加。36个月后，正常皮肤和HS中核心蛋白聚糖含量无差异（P>0.05）。此外，HS组织中mRNA表达水平在6个月内低于正常皮肤，但从7至12个月开始增加。在13至36个月期间mRNA表达持续增加，之后恢复到与正常皮肤相似的水平。
增生性瘢痕中核心蛋白聚糖的减少主要是由于合成减少所致。核心蛋白聚糖水平的增加与瘢痕组织稳定的时间一致，这表明其延迟出现与HS的形成相关。
