Miki Tsuyoshi, Okada Nobuhiko, Shimada Yukie, Danbara Hirofumi
Department of Microbiology, School of Pharmaceutical Sciences, Kitasato University, 5-9-1 Shirokane, Minato-Ku, Tokyo 108-8641, Japan.
Microb Pathog. 2004 Aug;37(2):65-72. doi: 10.1016/j.micpath.2004.04.006.
A number of bacteria that are pathogenic for animals and plants possess a type III secretion system (TTSS) to translocate virulence-associated proteins into host cells. In several bacteria, it has been reported that the TTSS is correlated with an ability to cause contact-dependent hemolysis in vitro. Here, we showed that the Salmonella enterica serovar Typhimurium strain SL1344 exhibited Salmonella pathogenicity island 1 type III secretion-dependent, contact-mediated, hemolytic activity. Mutations with a single deletion in genes encoding putative pore-forming proteins, SipB and SipC, secreted by the TTSS abolished hemolytic activity. In addition, the osmoprotection studies revealed that molecules larger than PEG2000 conferred significant protection against lysis induced by Salmonella. These results indicate that the hemolysis generated by Salmonella is due to the formation of pores within the erythrocyte membrane.
许多对动植物致病的细菌拥有III型分泌系统（TTSS），可将毒力相关蛋白转运到宿主细胞中。在几种细菌中，已有报道称TTSS与体外引起接触依赖性溶血的能力相关。在此，我们表明鼠伤寒沙门氏菌血清型鼠伤寒菌株SL1344表现出沙门氏菌致病岛1型III型分泌依赖性、接触介导的溶血活性。由TTSS分泌的编码假定成孔蛋白SipB和SipC的基因中单个缺失的突变消除了溶血活性。此外，渗透保护研究表明，大于PEG2000的分子对沙门氏菌诱导的裂解具有显著的保护作用。这些结果表明，沙门氏菌产生的溶血是由于红细胞膜内形成了孔。
