Antunes Eduardo N F, Ferreira Eliane O, Falcão Laís S, Paula Geraldo R, Avelar Katia E S, Barroso David E, Leite José Paulo G, Ferreira Maria Candida S, Domingues Regina M C P
Departamento de Microbiologia Médica, Instituto de Microbiologia, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, Ilha do Fundão, 21941-590 Rio de Janeiro, RJ, Brazil.
Res Microbiol. 2004 Sep;155(7):522-4. doi: 10.1016/j.resmic.2004.04.008.
Diarrhoeic stool samples from 334 0-5-year-old children were analysed with respect to the incidence of Bacteroides fragilis as well as other enteropathogens. B. fragilis was recovered in 9.3% (31/334) of the samples, and 79 strains were examined for the presence of the bft gene or the BfPAI flanking region using polymerase chain reaction assays. No enterotoxigenic B. fragilis strains were detected. In 29% (9/31) of the samples the coexistence of both II and III non-toxigenic B. fragilis (NTBF) patterns could be seen. In 51.6% (16/31) of the samples there existed a pattern II NTBF only, and in 19.4% (6/31) only pattern III could be detected. Strains from the same patient representing different patterns were submitted to pulsed-field gel electrophoresis assays. Fingerprints obtained by this technique showed that there was strong heterogeneity among strains from different individuals. However, different patterns from the same individual shared 100% similarity.
对334份0至5岁儿童的腹泻粪便样本进行了分析，以研究脆弱拟杆菌以及其他肠道病原体的发生率。在9.3%（31/334）的样本中检测到脆弱拟杆菌，使用聚合酶链反应分析法对79株菌株检测bft基因或脆弱拟杆菌致病相关基因岛（BfPAI）侧翼区域的存在情况。未检测到产肠毒素的脆弱拟杆菌菌株。在29%（9/31）的样本中可观察到II型和III型非产毒脆弱拟杆菌（NTBF）模式共存。在51.6%（16/31）的样本中仅存在II型NTBF模式，在19.4%（6/31）的样本中仅检测到III型模式。来自同一患者的代表不同模式的菌株进行了脉冲场凝胶电泳分析。通过该技术获得的指纹图谱显示，不同个体的菌株之间存在很强的异质性。然而，来自同一个体的不同模式具有100%的相似性。
