人血培养分离株中脆弱拟杆菌致病岛的特征分析。
Characterization of the Bacteroides fragilis pathogenicity island in human blood culture isolates.
作者信息
Claros M C, Claros Z C, Hecht D W, Citron D M, Goldstein E J C, Silva J, Tang-Feldman Y, Rodloff A C
机构信息
Institute of Medical Microbiology and Infectious Epidemiology, University of Leipzig, Germany.
出版信息
Anaerobe. 2006 Feb;12(1):17-22. doi: 10.1016/j.anaerobe.2005.06.005. Epub 2005 Aug 15.
Bacteroides fragilis is an important anaerobic pathogen accounting for up to 10% of bacteremias in adult patients. Enterotoxin producing B. fragilis (ETBF) strains have been identified as enteric pathogens of children and adults. In order to further characterize the B. fragilis pathogenicity island (BfPAI) and using PCR assays for bft- and mpII-metalloprotease genes, we determined the frequency of B. fragilis strains with pattern I (containing the BfPAI and its flanking region), pattern II (lacking both the BfPAI and the flanking region), and pattern III (lacking the BfPAI but containing the flanking region) in 63 blood culture isolates. The results were compared to 197 B. fragilis isolates from different clinical sources. We found 19% of blood culture isolates were pattern I (ETBF), 43% were pattern II (NTBF) and 38% were pattern III (NTBF). Comparatively, B. fragilis isolates from other clinical sources were 10% pattern I, 47% pattern II and 43% pattern III. This suggests that the pathogenicity island and the flanking elements may be general virulence factors of B. fragilis.
脆弱拟杆菌是一种重要的厌氧病原体，在成年患者的菌血症中占比高达10%。产肠毒素脆弱拟杆菌（ETBF）菌株已被确认为儿童和成人的肠道病原体。为了进一步表征脆弱拟杆菌致病岛（BfPAI），并使用针对bft和mpII金属蛋白酶基因的PCR检测方法，我们确定了63株血培养分离株中具有模式I（包含BfPAI及其侧翼区域）、模式II（既缺乏BfPAI也缺乏侧翼区域）和模式III（缺乏BfPAI但包含侧翼区域）的脆弱拟杆菌菌株的频率。将结果与来自不同临床来源的197株脆弱拟杆菌分离株进行了比较。我们发现19%的血培养分离株为模式I（ETBF），43%为模式II（NTBF），38%为模式III（NTBF）。相比之下，来自其他临床来源的脆弱拟杆菌分离株中，模式I占10%，模式II占47%，模式III占43%。这表明致病岛及其侧翼元件可能是脆弱拟杆菌的一般毒力因子。
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