Food and Nutrition Academic and Research Cluster, Institute of Nutrition, Mahidol University, Nakhon Pathom 73170, Thailand.
Molecules. 2024 Feb 28;29(5):1050. doi: 10.3390/molecules29051050.
L., a parasitic root in the Orobanchaceae family, is used as a food colorant in traditional Thai desserts. However, scant information is available on its food applications as well as medicinal properties, while overharvesting by the local people has severely depleted wild plant populations. This research, thus, aimed to extract optimized total phenolic content (TPC) in varying extraction conditions using response surface methodology (RSM) and the Box-Behnken design (BBD). Results indicated that an extraction temperature of 90 °C, 80% (/) aqueous ethanol, and 0.5% (/) solid-to-liquid ratio yielded the highest TPC at 129.39 mg gallic acid equivalent (GAE)/g dry weight (DW). Liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) identified the predominant phenolics as apigenin (109.06 mg/100 g extract) and luteolin (35.32 mg/100 g extract) with trace amounts of naringenin and rutin. Under the optimal extraction condition, the plant extract exhibited antioxidant activities of 5620.58 and 641.52 µmol Trolox equivalent (TE)/g DW determined by oxygen radical absorbance capacity (ORAC) and ferric ion reducing antioxidant power (FRAP) assay, while the scavenging capacity of total radicals at 50% (SC) was determined to be 135.50 µg/mL using 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay. The plant extract also exhibited inhibitory activities against the key enzymes relevant to type II diabetes, obesity, and Alzheimer's disease, suggesting the potential for medicinal applications.
L. 是列当科的一种寄生根，在传统的泰国甜点中被用作食用色素。然而，关于它的食品应用和药用特性的信息很少，而当地人的过度采集已经严重耗尽了野生植物种群。因此，本研究旨在使用响应面法（RSM）和 Box-Behnken 设计（BBD）优化不同提取条件下的总酚含量（TPC）。结果表明，在提取温度为 90°C、80%（/）水乙醇和 0.5%（/）固液比的条件下，TPC 最高，为 129.39mg 没食子酸当量（GAE）/g 干重（DW）。液相色谱-电喷雾串联质谱（LC-ESI-MS/MS）鉴定出的主要酚类物质为芹菜素（109.06mg/100g 提取物）和木犀草素（35.32mg/100g 提取物），并含有微量的柚皮苷和芦丁。在最佳提取条件下，植物提取物的抗氧化活性分别为 5620.58 和 641.52µmol Trolox 当量（TE）/g DW，通过氧自由基吸收能力（ORAC）和铁离子还原抗氧化能力（FRAP）测定；而通过 2,2-二苯基-1-苦基肼（DPPH）自由基清除测定，总自由基清除率（SC）为 135.50µg/mL。植物提取物还对与 II 型糖尿病、肥胖症和阿尔茨海默病相关的关键酶表现出抑制活性，表明其具有药用潜力。
