Bucht A, Oksenberg J R, Lindblad S, Grönberg A, Steinman L, Klareskog L
Department of Clinical Immunology, Uppsala University Hospital, Sweden.
Scand J Immunol. 1992 Feb;35(2):159-65. doi: 10.1111/j.1365-3083.1992.tb02846.x.
With the aim of investigating the distribution of T cells expressing different T-cell receptors (TCR) in the inflamed synovial tissue of rheumatoid arthritis patients, we have used the polymerase chain reaction to amplify TCR V alpha and V beta transcripts from synovial biopsies obtained by arthroscopy from patients with arthritis of variable duration. From each of nine patients a single biopsy was taken. Southern hybridization analysis of amplified products revealed extensive heterogeneity of TCR V beta in most patients. On the other hand, restriction in V alpha gene expression was seen in several patients. A highly restricted V alpha repertoire was observed in all cases with arthritis of short duration. In addition, two of three samples of short duration yielded a more limited number of V beta transcripts than the others. No conformity was, however, seen in usage of individual V alpha and V beta transcripts among the investigated patients. The present data thus demonstrate variability in synovial TCR expression between rheumatoid arthritis patients, but they also indicate a development towards greater diversity with increasing disease duration, implicating the necessity for careful choice of cases, preferentially selecting for early stages of disease, when further analysing rheumatoid synovial T cells for TCR usage as well as for antigen specificity.
为了研究表达不同T细胞受体（TCR）的T细胞在类风湿性关节炎患者炎症滑膜组织中的分布，我们使用聚合酶链反应从通过关节镜检查从病程各异的关节炎患者获取的滑膜活检样本中扩增TCR Vα和Vβ转录本。从9名患者中每人获取一份活检样本。对扩增产物的Southern杂交分析显示，大多数患者的TCR Vβ存在广泛的异质性。另一方面，在一些患者中观察到Vα基因表达受限。在所有病程短的关节炎病例中均观察到高度受限的Vα库。此外，病程短的三个样本中有两个产生的Vβ转录本数量比其他样本少。然而，在所研究的患者中，个体Vα和Vβ转录本的使用情况并无一致性。因此，目前的数据表明类风湿性关节炎患者滑膜TCR表达存在变异性，但也表明随着病程延长多样性增加，这意味着在进一步分析类风湿性滑膜T细胞的TCR使用情况以及抗原特异性时，必须谨慎选择病例，优先选择疾病早期阶段的病例。
