Struyk L, Kurnick J T, Hawes G E, van Laar J M, Schipper R, Oksenberg J R, Steinman L, de Vries R R, Breedveld F C, van den Elsen P
Department of Immunohematology and Bloodbank, Leiden University Hospital, The Netherlands.
Hum Immunol. 1993 Aug;37(4):237-51. doi: 10.1016/0198-8859(93)90507-w.
In this study we analyzed the usage frequencies of the TCR V-gene segments by alpha beta+ T cells present in synovial fluid of 17 patients with chronic arthritis, including rheumatoid arthritis. The results of this study, obtained from semiquantitative PCR analyses, showed that in all patients most of the TCR V alpha- and V beta-gene segments could be detected both in fresh PBMCs and in fresh SFMCs. The relative frequencies of use of these V-region genes were variable between the different patients. Although there was some skewing of increased usage frequencies of particular TCR V alpha and V beta genes among SFMC-derived TCRs when compared with PBMCs, we could not correlate such increased TCR V-gene usage with the inflammation in the joints as a disease-specific marker.
在本研究中，我们分析了17例慢性关节炎患者（包括类风湿关节炎患者）滑液中存在的αβ + T细胞对TCR V基因片段的使用频率。这项研究通过半定量PCR分析得出的结果表明，在所有患者中，大多数TCR Vα和Vβ基因片段在新鲜外周血单个核细胞（PBMCs）和新鲜滑膜液单个核细胞（SFMCs）中均可检测到。这些V区基因的相对使用频率在不同患者之间存在差异。尽管与PBMCs相比，SFMC衍生的TCR中特定TCR Vα和Vβ基因的使用频率有所增加，但我们无法将这种TCR V基因使用频率的增加与关节炎症作为疾病特异性标志物相关联。
