Tomczykowska Małgorzata, Bielak Janusz, Bodys Artur, Drazkiewicz Piotr
Department of Internal Diseases and Endocrynology Cardinal Stefan Wyszyński Specialist Hospital in Lublin, Lublin, Poland.
Ann Univ Mariae Curie Sklodowska Med. 2003;58(1):11-4.
The aim of the study was to assess the oxidative metabolism of the unstimulated and zymosan-stimulated neutrophils expressed as the production of superoxide anion radicals, and to determine the plasma O2 levels in the patients with initial "outside-hospital" pneumonia. The study included 20 patients with the initial symptoms of "outside-hospital" pneumonia. The plasma erythrocytes and leukocytes were isolated from the basilic vein blood collected in fasting state, and the preparation containing over 99% of neutrophils was prepared. The amount of superoxide anion radicals produced by neutrophils and their plasma levels were determined using the method of cytochrome reduction--c 5x10(-5) M/l. The patients with initial pneumonia show increased oxidative metabolism demonstrated by higher production of superoxide anion radicals by neutrophils and by elevated plasma O2 levels. Determinations of the plasma superoxide anion radical levels in pneumonia patients may be a practical and easily accessible indicator of the activation of neutrophil oxidative metabolism.
本研究的目的是评估未受刺激和经酵母聚糖刺激的中性粒细胞的氧化代谢，以超氧阴离子自由基的产生来表示，并测定初发“院外”肺炎患者的血浆O2水平。该研究纳入了20例有“院外”肺炎初始症状的患者。从空腹状态下采集的贵要静脉血中分离出血浆红细胞和白细胞，并制备出中性粒细胞含量超过99%的制剂。使用细胞色素还原法（细胞色素c 5×10⁻⁵ M/l）测定中性粒细胞产生的超氧阴离子自由基量及其血浆水平。初发肺炎患者表现出氧化代谢增加，表现为中性粒细胞产生超氧阴离子自由基增多以及血浆O2水平升高。测定肺炎患者血浆超氧阴离子自由基水平可能是中性粒细胞氧化代谢激活的一个实用且易于获取的指标。
