Lai Eric Y C, Chyau Charng-Cherng, Mau Jeng-Leun, Chen Chien-Chou, Lai Yi-Jui, Shih Ching-Fang, Lin Long-Liu
Department of Nursing, Hungkuang University, Taichung 433-2, Taiwan.
Am J Chin Med. 2004;32(2):281-90. doi: 10.1142/S0192415X0400193X.
The chemical compositions of the essential oil of Curcuma zedoaria (Berg.) Rosc. were analyzed by gas chromatography-mass spectrometry (GC-MS) and showed a high content of epicurzerenone and curdione representing 46.6% and 13.7% of the total oil, respectively. The essential oil was evaluated for potential antimicrobial activity against Staphylococcus aureus, Escherichia coli, Pseudomonasa aeruginosa, Vibrio parahaemolyticus, Salmonella typhimurium and Bacillus cereus. V. parahaemolyticus was sensitive to the presence of the essential oil, while the most resistant strain appeared to be E. coli. Based on 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyl-tetrazolium bromide (MTT) assay, nitroblue tetrazolium (NBT) reduction and cell morphology, the essential oil of C. zedoaria could inhibit the proliferation of human promyelocytic leukemia HL-60 cells. These results suggest that the essential oil has the antimicrobial activity against some of Gram- positive and negative pathogenic microorganisms and the components of the extract lead to the apoptosis of human cancer cell line.
采用气相色谱-质谱联用（GC-MS）法分析了莪术（Curcuma zedoaria (Berg.) Rosc.）挥发油的化学成分，结果表明，莪术二酮和莪术烯二酮含量较高，分别占挥发油总量的46.6%和13.7%。对该挥发油针对金黄色葡萄球菌、大肠杆菌、铜绿假单胞菌、副溶血性弧菌、鼠伤寒沙门氏菌和蜡样芽孢杆菌的潜在抗菌活性进行了评估。副溶血性弧菌对挥发油敏感，而最具抗性的菌株似乎是大肠杆菌。基于3-(4,5-二甲基噻唑-2-基)-2,5-二苯基溴化四氮唑（MTT）法、氮蓝四唑（NBT）还原法和细胞形态学观察，莪术挥发油可抑制人早幼粒细胞白血病HL-60细胞的增殖。这些结果表明，该挥发油对一些革兰氏阳性和阴性致病微生物具有抗菌活性，且提取物的成分可导致人癌细胞系凋亡。
