Plouraboue F, Cloetens P, Fonta C, Steyer A, Lauwers F, Marc-Vergnes J P
I.M.F.T., UMR 5502, Allées du Pr. C Soula, 31400 Toulouse, France.
J Microsc. 2004 Aug;215(Pt 2):139-48. doi: 10.1111/j.0022-2720.2004.01362.x.
This paper presents the first application of high-resolution X-ray synchrotron tomography to the imaging of large microvascular networks in biological tissue samples. This technique offers the opportunity of analysing the full three-dimensional vascular network from the micrometre to the millimetre scale. This paper presents the specific sample preparation method and the X-ray imaging procedure. Either barium or iron was injected as contrast agent in the vascular network. The impact of the composition and concentration of the injected solution on the X-ray synchrotron tomography images has been studied. Two imaging modes, attenuation and phase contrast, are compared. Synchrotron high-resolution computed tomography offers new prospects in the three-dimensional imaging of in situ biological vascular networks.
本文介绍了高分辨率X射线同步辐射断层扫描技术在生物组织样本中大微血管网络成像方面的首次应用。该技术提供了从微米级到毫米级分析完整三维血管网络的机会。本文介绍了具体的样本制备方法和X射线成像程序。在血管网络中注入钡或铁作为造影剂。研究了注入溶液的成分和浓度对X射线同步辐射断层扫描图像的影响。比较了衰减和相衬两种成像模式。同步辐射高分辨率计算机断层扫描为原位生物血管网络的三维成像提供了新的前景。
