嗜酸性粒细胞过氧化物酶基因单核苷酸多态性与日本雪松花粉症的关联
Association of single nucleotide polymorphisms in the eosinophil peroxidase gene with Japanese cedar pollinosis.
作者信息
Nakamura Hiroyuki, Higashikawa Fumiko, Miyagawa Kiyoshi, Nobukuni Yoshitaka, Endo Tomohiko, Imai Toru, Ozasa Kotaro, Motohashi Yutaka, Matsuzaki Ichiyo, Sasahara Shinichiro, Hatta Kotaro, Ogino Keiki, Eboshida Akira
机构信息
Department of Environmental Medicine, Kochi University School of Medicine, Kohasu, Okoh-cho, Nankoku 783-8505, Japan.
出版信息
Int Arch Allergy Immunol. 2004 Sep;135(1):40-3. doi: 10.1159/000080222. Epub 2004 Aug 13.
BACKGROUND
Japanese cedar pollinosis is the most common form of hay fever in spring in Japan. We have previously demonstrated that single nucleotide polymorphism Pro358Leu of exon 7 in the eosinophil peroxidase (EPO) gene is associated with cedar pollinosis, although the association has not been confirmed by analysis of the whole gene in a different population.
METHODS
We sequenced all exons of the EPO gene in 60 children with pollinosis and their parents using the PCR-restriction fragment length polymorphism method.
RESULTS
We found 8 polymorphisms, Ile40Met, Gln122His, Arg202Arg (A660G), Asn303Asn (C909T), Arg326Pro, Arg326His, Pro358Leu, and Asn572Ty, in the EPO gene. As a result of the transmission disequilibrium test, we recognized significant transmissions of 202Arg (660G) in exon 6 in addition to 358Leu of exon 7 in the EPO gene of affected children.
CONCLUSIONS
Our results might indicate that polymorphisms of the EPO gene are associated with Japanese cedar pollinosis.
背景
日本雪松花粉症是日本春季最常见的花粉热形式。我们之前已经证明，嗜酸性粒细胞过氧化物酶（EPO）基因第7外显子的单核苷酸多态性Pro358Leu与雪松花粉症有关，尽管在不同人群中对整个基因进行分析尚未证实这种关联。
方法
我们使用聚合酶链反应-限制性片段长度多态性方法对60名花粉症儿童及其父母的EPO基因所有外显子进行测序。
结果
我们在EPO基因中发现了8种多态性，即Ile40Met、Gln122His、Arg202Arg（A660G）、Asn303Asn（C909T）、Arg326Pro、Arg326His、Pro358Leu和Asn572Ty。通过传递不平衡检验，我们发现除了患病儿童EPO基因第7外显子的358Leu外，第6外显子的202Arg（660G）也存在显著传递。
结论
我们的结果可能表明EPO基因多态性与日本雪松花粉症有关。
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