[外伤性鼓膜穿孔。在动物模型中局部应用负载表皮生长因子的藻酸盐基质]

[Traumatic tympanic membrane perforactions. Local application of an alginate matrix loaded with epidermal growth factor in an animal model].

作者信息

Kaftan H, Hosemann W, Junghans D, Göpferich A, Schindler E, Beule A

机构信息

Klinik und Poliklinik für Hals-, Nasen- und Ohrenkrankheiten, Kopf- und Hals-Chirurgie der Ernst-Moritz-Arndt-Universität Greifswald.

出版信息

HNO. 2005 Jun;53(6):539-42, 544-5. doi: 10.1007/s00106-004-1126-9.

DOI:10.1007/s00106-004-1126-9

PMID:15316628

Abstract

BACKGROUND

The aim of this study was to evaluate the effects of an alginate matrix releasing epidermal growth factor on healing after acute tympanic membrane perforation in rats.

METHOD

A total of 20 male rats were divided into two groups. In each animal, a randomly chosen tympanic membrane was perforated by heat. A piece of alginate matrix (control group) or alginate matrix loaded with 0.25 microg epidermal growth factor (EGF group) was then placed on the perforation. The rat ears were examined after days 3, 6, 9, and 14 and every week thereafter for a total of 11 weeks. Each matrix was removed on day 9. To examine the status of the tympanic membranes on day 14, one randomly chosen membrane from each group was histopathologically examined.

RESULTS

By day 6, complete closure of the tympanic membrane perforation was achieved in 56% of the EGF group, whereas it was achieved in only 10% in the control group. By day 14, all tympanic membrane perforations were closed in both groups. There were no complications and no significant differences in the histopathologic parameters between the EGF group and the control group.

CONCLUSION

An alginate matrix seems to be a useful EGF-delivery system to the tympanic membrane.

摘要

背景

本研究旨在评估释放表皮生长因子的藻酸盐基质对大鼠急性鼓膜穿孔后愈合的影响。

方法

将20只雄性大鼠分为两组。在每只动物中，随机选择一侧鼓膜进行热穿孔。然后将一片藻酸盐基质（对照组）或负载0.25微克表皮生长因子的藻酸盐基质（表皮生长因子组）放置在穿孔处。在第3、6、9和14天以及此后每周对大鼠耳朵进行检查，共检查11周。在第9天取出每个基质。为了检查第14天鼓膜的状态，从每组中随机选择一个鼓膜进行组织病理学检查。