San Vicente Blanca, Navarro Villoslada Fernando, Moreno-Bondi María C
Laboratory of Optical Sensors, Department of Analytical Chemistry, Faculty of Chemistry, Universidad Complutense de Madrid, 28040 Madrid, Spain.
Anal Bioanal Chem. 2004 Sep;380(1):115-22. doi: 10.1007/s00216-004-2744-5. Epub 2004 Aug 13.
A bisphenol A (BPA) molecularly imprinted polymer, the composition of which was optimised using a chemometric approach, has been applied to the selective preconcentration of the template from aqueous samples. The selectivity of the polymer toward BPA and related compounds was evaluated chromatographically. The BPA-imprinted polymer was packed in a column and used for continuous on-column solid-phase extraction (MISPE) of aqueous samples followed by subsequent analysis by HPLC with fluorescence detection of the eluted fractions. The composition of the washing solvent applied in the MISPE procedure was optimised to favour the specific interactions of the MIP with BPA and to remove the non-selectively bound matrix components. The MISPE method has proven to be effective for selective preconcentration of BPA in aqueous samples (recoveries >84% obtained in the eluate for 10-100 mL sample volumes) enabling detection and quantification limits of 1.0 and 3.3 ng mL(-1), respectively (based on 25 mL sample size). Analytical recoveries were between 92 and 101% for river water samples spiked with known amounts of BPA (30, 60, and 80 ng mL(-1)); relative standard deviations (RSD) were lower than 5.0%.
一种采用化学计量学方法优化了组成的双酚A（BPA）分子印迹聚合物，已应用于从水样中选择性预富集模板。通过色谱法评估了该聚合物对BPA及相关化合物的选择性。将BPA印迹聚合物填充到柱中，用于水样的连续柱上固相萃取（MISPE），随后通过高效液相色谱法对洗脱馏分进行荧光检测分析。对MISPE过程中使用的洗涤溶剂的组成进行了优化，以促进MIP与BPA的特异性相互作用，并去除非选择性结合的基质成分。事实证明，MISPE方法对于水样中BPA的选择性预富集是有效的（对于10 - 100 mL的样品体积，洗脱液中的回收率>84%），检测限和定量限分别为1.0和3.3 ng mL⁻¹（基于25 mL的样品量）。对于添加了已知量BPA（30、60和80 ng mL⁻¹）的河水样品，分析回收率在92%至101%之间；相对标准偏差（RSD）低于5.0%。
