Wu Ya-Ting, Zhang Yan-Hong, Zhang Meng, Liu Fei, Wan Ying-Chun, Huang Zheng, Ye Lei, Zhou Qi, Shi Yun, Lu Bin
Key Laboratory of Environment and Health, Ministry of Education & Ministry of Environmental Protection, and State Key Laboratory of Environmental Health (Incubating), School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China.
Key Laboratory of Environment and Health, Ministry of Education & Ministry of Environmental Protection, and State Key Laboratory of Environmental Health (Incubating), School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China; Pure and Applied Biochemistry, Center for Chemistry and Chemical Engineering, Lund University, Box 124, SE 22 100 Lund, Sweden.
Food Chem. 2014 Dec 1;164:527-35. doi: 10.1016/j.foodchem.2014.05.071. Epub 2014 May 21.
Nanofibrous molecularly imprinted membranes (nano-MIMs) with multi-analyte selectivity were prepared by encapsulating two types of molecularly imprinted polymer nanoparticles (MIP-NPs) into electrospun polyvinyl alcohol nanofibers. The obtained nano-MIMs maintained high molecular selectivity offered by each of the MIP-NPs. Nano-MIM embedding BPA-imprinted nanoparticles and TBZ-imprinted nanoparticles together showed the highest binding selectivity for acid bisphenol A (BPA) and basic tebuconazole (TBZ). This nano-MIM was used as affinity material of membrane-based molecularly imprinted solid-phase extraction (m-MISPE) to extract trace BPA and TBZ in vegetables and juices simultaneously. The recoveries of BPA and TBZ from different samples were higher than 70.33% with RSDs lower than 9.57%. m-MISPE gave better HPLC separation efficiencies and higher recoveries than conventional SPE based on C18/SCX. Multi-analyte selective m-MISPE combined with HPLC realized selective and simultaneous determination of several trace analytes with opposite charges/polarities in different food samples.
通过将两种分子印迹聚合物纳米颗粒（MIP-NPs）封装到静电纺丝聚乙烯醇纳米纤维中，制备了具有多分析物选择性的纳米纤维分子印迹膜（nano-MIMs）。所得的nano-MIMs保持了每种MIP-NPs提供的高分子选择性。同时嵌入双酚A（BPA）印迹纳米颗粒和噻菌灵（TBZ）印迹纳米颗粒的nano-MIM对酸性双酚A（BPA）和碱性噻菌灵（TBZ）表现出最高的结合选择性。这种nano-MIM用作基于膜的分子印迹固相萃取（m-MISPE）的亲和材料，用于同时萃取蔬菜和果汁中的痕量BPA和TBZ。不同样品中BPA和TBZ的回收率高于70.33%，相对标准偏差低于9.57%。与基于C18/SCX的传统固相萃取相比，m-MISPE具有更好的高效液相色谱分离效率和更高的回收率。多分析物选择性m-MISPE与高效液相色谱相结合，实现了对不同食品样品中几种带相反电荷/极性的痕量分析物的选择性同时测定。
