Goodier M, Fey P, Eichmann K, Langhorne J
Max-Planck-Institut für Immunbiologie, Freiburg, Germany.
Int Immunol. 1992 Jan;4(1):33-41. doi: 10.1093/intimm/4.1.33.
Peripheral blood lymphocytes from donors previously unexposed to malaria parasites proliferate in vitro when stimulated with whole parasitized red blood cells of several different strains of Plasmodium falciparum. Here we show that both cells enriched for both memory (CD45R0+) and naïve (CD45R0-) phenotype can respond. Cells involved in these responses occur at frequencies similar to those observed for recall antigens such as tetanus toxoid but at lower frequencies than observed for the superantigens staphylococcal enterotoxin B or the mitogenic lectin phytohemagglutinin (PHA). Proliferation is inhibited by antibodies to class II MHC and to CD3 molecules. Stimulation of purified CD45R0- T cells by whole parasitized red blood cells for 6 days results in the generation of a large proportion of gamma delta T cell blasts of V gamma 9V delta 2 TCR phenotype and in the acquisition of the CD45R0 molecule within the blast cell population. The rapid generation of a vigorous primary in vitro gamma delta T cell response by malarial parasites may reflect the situation during primary malarial infection.
来自此前未接触过疟原虫的供体的外周血淋巴细胞，在受到几种不同恶性疟原虫株的完整寄生红细胞刺激时，会在体外增殖。我们在此表明，富集了记忆（CD45R0+）和初始（CD45R0-）表型的细胞均可产生反应。参与这些反应的细胞出现的频率与针对诸如破伤风类毒素等回忆抗原所观察到的频率相似，但低于针对超抗原葡萄球菌肠毒素B或促有丝分裂凝集素植物血凝素（PHA）所观察到的频率。增殖受到针对II类MHC和CD3分子的抗体的抑制。完整寄生红细胞对纯化的CD45R0- T细胞刺激6天，会导致产生很大比例的Vγ9Vδ2 TCR表型的γδ T细胞母细胞，并使母细胞群体中获得CD45R0分子。疟原虫在体外迅速产生强烈的初始γδ T细胞反应，这可能反映了初次疟疾感染期间的情况。
