Goerlich R, Häcker G, Pfeffer K, Heeg K, Wagner H
Institute of Medical Microbiology and Hygiene, Technical University of Munich, FRG.
Eur J Immunol. 1991 Oct;21(10):2613-6. doi: 10.1002/eji.1830211045.
Peripheral blood-derived T cells from six unprimed caucasian donors were tested for the in vitro reactivity to Plasmodium falciparum merozoites (PFM). Without exception vigorous proliferative responses were observed within the donors tested. The frequency of PFM-reactive T cells ranged from 1/150-1/300. Phenotypic analysis of peripheral blood lymphocytes cultured in the presence of PFM revealed the preferential outgrowth of gamma/delta T cells, which represented within 7 days about 70% of the reactive T cell blasts. All reactive gamma/delta T cell blasts displayed the V gamma 9+ TcR phenotype. We conclude that human gamma/delta T cells respond vigorously to PFM, and that this property is confined to V gamma 9+ T cell subset.
对来自六名未接触过疟原虫的白种人供体的外周血来源的T细胞进行了针对恶性疟原虫裂殖子（PFM）的体外反应性测试。在所测试的供体中无一例外都观察到了强烈的增殖反应。PFM反应性T细胞的频率范围为1/150 - 1/300。在PFM存在的情况下培养的外周血淋巴细胞的表型分析显示γ/δ T细胞优先生长，在7天内约占反应性T细胞母细胞的70%。所有反应性γ/δ T细胞母细胞均表现出Vγ9 + TcR表型。我们得出结论，人类γ/δ T细胞对PFM有强烈反应，并且这种特性仅限于Vγ9 + T细胞亚群。
