含有2'-氨基乙氧基-5-(3-氨基丙-1-炔基)尿苷的三链形成寡核苷酸识别双链DNA中AT碱基对的选择性和亲和力。
Selectivity and affinity of triplex-forming oligonucleotides containing 2'-aminoethoxy-5-(3-aminoprop-1-ynyl)uridine for recognizing AT base pairs in duplex DNA.
作者信息
Osborne Sadie D, Powers Vicki E C, Rusling David A, Lack Oliver, Fox Keith R, Brown Tom
机构信息
School of Chemistry, University of Southampton, Highfield, Southampton SC17 1BJ, UK.
出版信息
Nucleic Acids Res. 2004 Aug 18;32(15):4439-47. doi: 10.1093/nar/gkh776. Print 2004.
Abstract
We have used DNase I footprinting, fluorescence and ultraviolet (UV) melting experiments and circular dichroism to demonstrate that, in the parallel triplex binding motif, 2'-aminoethoxy-5-(3-aminoprop-1-ynyl)uridine (bis-amino-U, BAU) has very high affinity for AT relative to all other Watson-Crick base pairs in DNA. Complexes containing two or more substitutions with this nucleotide analogue are stable at pH 7.0, even though they contain several C.GC base triplets. These modified triplex-forming oligonucleotides retain exquisite sequence specificity, with enhanced discrimination against YR base pairs (especially CG). These properties make BAU a useful base analogue for the sequence-specific creation of stable triple helices at pH 7.0.
摘要
我们已运用DNA酶I足迹法、荧光和紫外（UV）熔解实验以及圆二色性来证明，在平行三链体结合基序中，相对于DNA中所有其他沃森-克里克碱基对，2'-氨基乙氧基-5-(3-氨基丙-1-炔基)尿苷（双氨基-U，BAU）对AT具有非常高的亲和力。含有两个或更多个这种核苷酸类似物取代的复合物在pH 7.0时是稳定的，尽管它们含有几个C.GC碱基三联体。这些经修饰的三链体形成寡核苷酸保留了精确的序列特异性，对YR碱基对（尤其是CG）的区分能力增强。这些特性使BAU成为在pH 7.0时用于序列特异性创建稳定三链螺旋的有用碱基类似物。
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