含 2'-O-(2-甲氧基乙基)-5-(3-氨丙基-1-炔基)-尿嘧啶的三链形成寡核苷酸的合成与性质。
Synthesis and properties of triplex-forming oligonucleotides containing 2'-O-(2-methoxyethyl)-5-(3-aminoprop-1-ynyl)-uridine.
机构信息
School of Chemistry, University of Southampton, Highfield, Southampton SO17 1BJ, UK.
出版信息
Bioorg Med Chem. 2010 Sep 1;18(17):6389-97. doi: 10.1016/j.bmc.2010.07.005. Epub 2010 Jul 30.
2'-O-(2-Methoxyethyl)-5-(3-aminoprop-1-ynyl)-uridine phosphoramidite (MEPU) has been synthesized from d-ribose and 5-iodouracil and incorporated into triplex-forming oligonucleotides (TFOs) by automated solid-phase oligonucleotide synthesis. The TFOs gave very high triplex stability with their target duplexes as measured by ultraviolet/fluorescence melting and DNase I footprinting. The incorporation of MEPU into TFOs renders them resistant to degradation by serum nucleases.
2'-O-(2-甲氧基乙基)-5-(3-氨基丙-1-炔基)-尿苷亚磷酰胺（MEPU）已由 D-核糖和 5-碘尿嘧啶合成，并通过自动化固相寡核苷酸合成掺入三链体形成寡核苷酸（TFO）。通过紫外/荧光解链和 DNase I 足迹法测量，TFO 与其靶双链体结合具有非常高的三链体稳定性。MEPU 掺入 TFO 可使其抵抗血清核酸酶的降解。
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