Ishizaki T, Itoh R, Yamamoto T, Okada H
Department of Obstetrics and Gynecology, Kyoto Prefectural University of Medicine.
Nihon Sanka Fujinka Gakkai Zasshi. 1992 Jan;44(1):67-72.
In order to establish a sensitivity test system for the evaluation of anti-cancer hormonal agents, we tried a long-term subrenal capsule (SRC) assay, using nude mice with a transplanted solid tumor of endometrial cancer cells (Ishikawa's line). Unlike DNA-affecting agents, anti-cancer hormonal agents exert cytostatic effects rather than cytocidal effects, and their evaluation in a short period is considered to be inaccurate. Our test system is somewhat difficult in terms of technique, but it is useful since it can (1) evaluate the agents in the relatively short period of 28 days and (2) compare the cytostatic anti-tumor efficacy of two or more agents under the same conditions. The rate of successful tumor transplantation in nude mice in our system is very high, i.e., more than 90%. Although there are some points which need improvement, our system is considered to be useful as an assay system for the development of anti-cancer hormonal agents and other similar chemotherapeutic agents for cancers. When medroxyprogesterone acetate (MPA) was evaluated by means of this system, the administration period, as well as the dosage, was found to be important.
为建立用于评估抗癌激素制剂的敏感性测试系统，我们尝试了长期肾包膜下（SRC）试验，使用移植了子宫内膜癌细胞（石川株）实体瘤的裸鼠。与影响DNA的制剂不同，抗癌激素制剂发挥的是细胞生长抑制作用而非细胞杀伤作用，并且认为在短时间内对它们进行评估是不准确的。我们的测试系统在技术方面有些难度，但它很有用，因为它可以（1）在相对较短的28天内评估制剂，以及（2）在相同条件下比较两种或更多种制剂的细胞生长抑制抗肿瘤功效。我们系统中裸鼠的肿瘤移植成功率非常高，即超过90%。尽管有一些需要改进的地方，但我们的系统被认为可作为开发抗癌激素制剂及其他类似癌症化疗药物的一种测试系统。当通过该系统评估醋酸甲羟孕酮（MPA）时，发现给药期以及剂量都很重要。
