Zeiler Thomas, Gritzka Debora, Karger Ralf, Kretschmer Volker
Institute for Transfusion Medicine and Haemostaseology, University Hospital, Philipps-University Marburg, Germany.
Transfusion. 2004 Sep;44(9):1300-5. doi: 10.1111/j.1537-2995.2004.03418.x.
Preventing the activation of PLTs may ameliorate (or mitigate) the PLT storage lesion (PSL), which encloses all structural and biochemical changes caused by collection, processing, and storage of PLT concentrates (PCs). Partial inhibition of PLT function due to ingestion of aspirin (ASA) by blood donors reduces the functional activity of the collected PLTs, however, by preventing premature PLT activation, it might reduce the PSL as well.
In a randomized crossover study, 10 healthy donors donated two single-donor PCs (SDPCs) each, taking 500 mg ASA 12 hours before one of the aphereses (Group A) and taking no medication before the other donation (Group B). In-vitro tests of PLT function were performed in donors before and after apheresis and in SDPCs during storage (Days 1, 3, and 5).
ASA ingestion resulted in a significant decrease of induced PLT aggregation in donors (p < 0.005) and SDPCs on Day 1 (p < 0.01). TRAP-6-induced expression of p-selectin (CD62p) was significantly reduced in Group A SDPCs only on Day 1 (p < 0.02). There were no significant differences of in-vitro function (LDH, lactate, pH, morphology score, CD62p expression, fibrinogen binding) between Group A and B (SDPCs and donors). Apheresis did not result in a significant activation of PLTs in donors or SDPCs.
These limited data do not show a detectable beneficial effect of ASA ingestion on the PSL but do suggest that ASA ingestion before apheresis may not be detrimental to the clinical effectiveness of the stored product.
防止血小板（PLT）激活可能会改善（或减轻）血小板储存损伤（PSL），PSL涵盖了血小板浓缩物（PCs）采集、处理和储存所引起的所有结构和生化变化。然而，献血者摄入阿司匹林（ASA）导致PLT功能部分抑制，这会降低所采集PLT的功能活性，但通过防止PLT过早激活，也可能会减轻PSL。
在一项随机交叉研究中，10名健康献血者每人捐献两份单采血小板（SDPCs），其中一次单采前12小时服用500 mg ASA（A组），另一次献血前不服药（B组）。在单采前后对献血者以及储存期间（第1、3和5天）的SDPCs进行PLT功能的体外检测。
摄入ASA导致献血者（p < 0.005）和第1天的SDPCs（p < 0.01）中诱导的PLT聚集显著降低。仅在第1天，A组SDPCs中TRAP-6诱导的p-选择素（CD62p）表达显著降低（p < 0.02）。A组和B组（SDPCs和献血者）之间的体外功能（乳酸脱氢酶、乳酸、pH值、形态学评分、CD62p表达、纤维蛋白原结合）无显著差异。单采未导致献血者或SDPCs中PLT的显著激活。
这些有限的数据未显示摄入ASA对PSL有可检测到的有益作用，但表明单采前摄入ASA可能不会损害储存产品的临床有效性。
