Herzberg G R, Chernenko G A, Barrowman J A, Kean K T, Keough K M
Department of Biochemistry, Memorial University of Newfoundland, St. John's, Canada.
Biochim Biophys Acta. 1992 Mar 4;1124(2):190-4. doi: 10.1016/0005-2760(92)90097-f.
A study was conducted to evaluate whether the composition of previous dietary fat affects the absorption and composition of lymph obtained after a meal of fish oil. Adult male Sprague-Dawley rats were fed diets containing either corn oil or fish oil (MaxEPA) for 2 weeks. They were then given intraduodenally a bolus of an emulsion of 0.5 ml of fish oil plus 0.5 ml of 20 mM sodium taurocholate. Intestinal lymph was collected from a cannula in the main intestinal lymph trunk for various times after oil administration. Rats proportion of the test dose fo fish oil than those fed corn oil. There was an effect of previous diet on the fatty acid composition of the lymph. Rats fed fish oil had a higher percentage of eicosapentaenoic and docosahexaenoic acids in the lymph lipids than those fed corn oil while those fed corn oil had a higher percentage of linoleic acid. These results rule out decreased intestinal absorption as a mechanism for the hypotriacylglycerolemic effect of dietary fish oils. They also indicate a significant contribution of endogenous lipids to the fatty acids in lymph.
开展了一项研究，以评估先前膳食脂肪的组成是否会影响进食鱼油后所获取淋巴液的吸收及组成。成年雄性斯普拉格-道利大鼠被喂食含玉米油或鱼油（MaxEPA）的日粮，为期2周。随后经十二指肠给它们注入0.5毫升鱼油与0.5毫升20毫摩尔牛磺胆酸钠乳剂的大剂量混合物。在注入油剂后的不同时间，从主肠淋巴干中的插管收集肠淋巴液。大鼠对鱼油测试剂量的吸收率高于喂食玉米油的大鼠。先前的饮食对淋巴液的脂肪酸组成有影响。喂食鱼油的大鼠淋巴脂质中二十碳五烯酸和二十二碳六烯酸的百分比高于喂食玉米油的大鼠，而喂食玉米油的大鼠淋巴脂质中亚油酸的百分比更高。这些结果排除了肠道吸收减少是膳食鱼油降三酰甘油作用机制的可能性。它们还表明内源性脂质对淋巴液中的脂肪酸有显著贡献。
