Degrace P, Caselli C, Rayo J M, Bernard A
Laboratoire de Physiologie de la Nutrition, Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation, Université de Bourgogne, Dijon, France.
Lipids. 1996 Apr;31(4):405-14. doi: 10.1007/BF02522927.
Adult male rats were surgically given a drainage catheter in the main mesenteric lymph duct. After an overnight fast, five groups of rats received intragastrically, in one bolus, butter, corn oil (CO), cod liver oil (CLO), menhaden oil (MO), or ethyl esters of eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids (K80). Intestinal lymph was collected in these conscious animals, each hour during the first 6 h and in a single sample for the next 18 h. The absorption peak appeared earlier after MO and CO than after CLO administration. The quantities of triglycerides recovered during the first 6 h were significantly lower after butter (91 mg) and K80 (54 mg) administration than for the other three oils. No difference was observed between the vegetable oil and the marine oils (CO = 173 mg, CLO = 148 mg, MO = 180 mg). The total triglyceride recovered in 24 h was highest after CLO (410 mg) and lowest with K80 (146 mg). An increase in the weight percentage of some characteristic fatty acids of the lipid mixtures was observed: oleic acid for butter, oleic and linoleic acids for CO, EPA and DHA for CLO, MO, and K80. Chylomicrons were the largest with CO, more numerous and smaller with CLO, and the smallest with K80. Results obtained illustrated the relation between gastrointestinal hydrolysis, enterocyte biochemical events, and lymph triglyceride absorption profiles as related to the composition and distribution of triglyceride fatty acids.
成年雄性大鼠通过手术在主肠系膜淋巴管中植入引流导管。禁食过夜后，五组大鼠分别一次性灌胃给予黄油、玉米油（CO）、鱼肝油（CLO）、鲱鱼油（MO）或二十碳五烯酸（EPA）和二十二碳六烯酸（DHA）的乙酯（K80）。在这些清醒的动物中收集肠淋巴液，最初6小时内每小时收集一次，接下来18小时收集一个样本。与给予CLO相比，给予MO和CO后吸收峰出现得更早。给予黄油（91毫克）和K80（54毫克）后，前6小时回收的甘油三酯量显著低于其他三种油。植物油和海洋油之间未观察到差异（CO = 173毫克，CLO = 148毫克，MO = 180毫克）。24小时内回收的总甘油三酯量在给予CLO后最高（410毫克），在给予K80后最低（146毫克）。观察到脂质混合物中一些特征性脂肪酸的重量百分比增加：黄油中的油酸、CO中的油酸和亚油酸、CLO、MO和K80中的EPA和DHA。乳糜微粒在CO组中最大，在CLO组中数量更多且更小，在K80组中最小。所得结果说明了胃肠道水解、肠细胞生化事件与肠淋巴甘油三酯吸收曲线之间与甘油三酯脂肪酸组成和分布的关系。
