噬菌体φX174晶体结构的初步研究。

Preliminary investigation of the phage phi X174 crystal structure.

作者信息

Willingmann P, Krishnaswamy S, McKenna R, Smith T J, Olson N H, Rossmann M G, Stow P L, Incardona N L

机构信息

Department of Biological Sciences, Purdue University, West Lafayette, IN 47907.

出版信息

J Mol Biol. 1990 Mar 20;212(2):345-50. doi: 10.1016/0022-2836(90)90129-a.

DOI:10.1016/0022-2836(90)90129-a

PMID:2138678

Abstract

Crystals of the single-stranded DNA bacteriophage phi X174 have been grown. They have a monoclinic unit cell with space group P2(1), unit cell dimensions of a = 306.0 (+/- 0.2) A, b = 361.1 (+/- 0.2) A, c = 299.7 (+/- 0.2 degrees) A, beta = 92.91 degrees (+/- 0.02 degrees) and diffract to at least 2.7 A resolution. There are two virus particles per unit cell. Packing considerations show that the mean diameter of the virus particles is 280 A. The virus separates into two bands in a sucrose gradient. The ratio between the absorbance at 260 nm and 280 nm is 1.45 to 1.65 for the faster and 1.15 to 1.35 for the slower bands, but both bands contain intact particles. Crystals derived from these bands are isomorphous and there is no detectable difference in their structure amplitudes.

摘要

单链DNA噬菌体φX174的晶体已经生长出来。它们具有单斜晶胞，空间群为P2(1)，晶胞参数为a = 306.0（±0.2）Å，b = 361.1（±0.2）Å，c = 299.7（±0.2°）Å，β = 92.91°（±0.02°），并且能衍射到至少2.7 Å的分辨率。每个晶胞中有两个病毒颗粒。堆积分析表明病毒颗粒的平均直径为280 Å。病毒在蔗糖梯度中分离成两条带。较快带在260 nm和280 nm处的吸光度之比为1.45至1.65，较慢带为1.15至1.35，但两条带都含有完整的颗粒。源自这些带的晶体是同晶型的，并且它们的结构振幅没有可检测到的差异。