Zhang Dongmao, Mrozek Melissa F, Xie Yong, Ben-Amotz Dor
Purdue University, Department of Chemistry, West Lafayette, Indiana 47907, USA.
Appl Spectrosc. 2004 Aug;58(8):929-33. doi: 10.1366/0003702041655430.
A new application of the recently described drop coating deposition Raman (DCDR) method facilitates the segregation and independent spectral characterization of mixture components. The quality of the normal (un-enhanced) Raman spectra are significantly improved as a result of reduced spectral interference from fluorescent impurities and buffer compounds. Fluorescence of commercial amino acid (O-phospho-L-serine) and protein (myoglobin) samples is reduced by over an order of magnitude using DCDR, more effectively than prolonged photo-bleaching. Furthermore, DCDR is used to obtain high-quality Raman spectra of proteins, lysozyme, and insulin, derived from solutions with up to 1000-fold excess buffer concentration. Possible thermodynamic and kinetic contributions to the observed segregation phenomena are discussed.
最近描述的滴涂沉积拉曼（DCDR）方法的一种新应用有助于混合物成分的分离和独立光谱表征。由于来自荧光杂质和缓冲化合物的光谱干扰减少，正常（未增强）拉曼光谱的质量得到显著提高。使用DCDR，商业氨基酸（O-磷酸-L-丝氨酸）和蛋白质（肌红蛋白）样品的荧光降低了一个数量级以上，比延长光漂白更有效。此外，DCDR用于从缓冲液浓度高达1000倍过量的溶液中获得蛋白质、溶菌酶和胰岛素的高质量拉曼光谱。讨论了观察到的分离现象可能的热力学和动力学贡献。
