Kliem Michele Ann, Wichmann Thomas
Department of Neurology, Emory University School of Medicine, Suite 6000, Woodruff Memorial Research Building, 101 Woodruff Circle, Atlanta, GA 30322, USA.
J Neurosci Methods. 2004 Sep 30;138(1-2):45-9. doi: 10.1016/j.jneumeth.2004.03.015.
A newly designed combination microelectrode-injection system is described which can be used to record electrophysiological responses of individual neurons in the primate brain to local administration of small quantities of drugs or other compounds. The assembly of the system is simple, and the materials used are inexpensive. The system consists of a standard tungsten microelectrode alongside fused silica tubing within a polyimide sleeve. The major advantage of this device is the ability to record with excellent quality, changes in the activity of single cells in vivo within 50-100 microm of the location of drug injections.
本文描述了一种新设计的组合式微电极-注射系统，该系统可用于记录灵长类动物大脑中单个神经元对局部施用少量药物或其他化合物的电生理反应。该系统组装简单，所用材料价格低廉。该系统由一个标准的钨微电极和聚酰亚胺套管内的熔融石英管组成。该装置的主要优点是能够在药物注射部位50-100微米范围内，高质量地记录体内单细胞活动的变化。
