Orro T, Sankari S, Pudas T, Oksanen A, Soveri T
Faculty of Veterinary Medicine, Department of Clinical Veterinary Sciences, University of Helsinki, P.O. BOX 57, 00014 Helsinki, Finland.
Comp Immunol Microbiol Infect Dis. 2004 Nov;27(6):413-22. doi: 10.1016/j.cimid.2004.01.005.
The serum concentrations of two acute phase proteins (APPs), haptoglobin (Hp) and serum amyloid-A (SAA), were monitored in reindeer after challenge with endotoxin. Four adult female reindeer received either 0.1 microg/kg Escherichia coli 0111:B4 lipopolysaccharide B or saline solution intravenously. At the second challenge, the treatments were reversed. In addition to the APPs, changes in blood chemistry and rectal temperature were monitored. The endotoxin challenge caused a significant increase in SAA (peak 48 h) and a sharp decrease (8-12 h) of serum iron concentrations in all animals. The mean Hp concentration increased at 8 h and remained elevated until 48 h, but no statistically significant differences were found. This investigation demonstrates that challenge with a single-bolus dose of E. coli endotoxin can activate the acute phase response (APR) and SAA appears to be a more sensitive indicator of the APR than Hp during bacterial infection in reindeer.
在用内毒素攻击驯鹿后，监测了两种急性期蛋白（APPs）——触珠蛋白（Hp）和血清淀粉样蛋白A（SAA）的血清浓度。四只成年雌性驯鹿静脉注射了0.1微克/千克的大肠杆菌0111:B4脂多糖B或生理盐水。在第二次攻击时，处理方式进行了互换。除了APPs外，还监测了血液化学成分和直肠温度的变化。内毒素攻击导致所有动物的SAA显著升高（峰值在48小时），血清铁浓度急剧下降（8 - 12小时）。平均Hp浓度在8小时时升高，并一直保持到48小时，但未发现统计学上的显著差异。这项研究表明，单次推注剂量的大肠杆菌内毒素攻击可激活急性期反应（APR），并且在驯鹿细菌感染期间，SAA似乎比Hp是APR更敏感的指标。
